Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)

Evgeni Eltzov, Avital Cohen, Robert S Marks

ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS

Product Number

Brand

Product Description

PHR1321

Supelco

Sodium chloride, Pharmaceutical Secondary Standard; Certified Reference Material

97622

Supelco

2-Mercaptoethanol, for HPLC derivatization, LiChropur^™, ≥99.0% (GC)

PHR1372

Supelco

Methanol, Pharmaceutical Secondary Standard; Certified Reference Material

M1775

Sigma-Aldrich

Methanol, Absolute - Acetone free

179337

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

178810

Sigma-Aldrich

Tetrahydrofuran, ReagentPlus^®, ≥99.0%, contains 250 ppm BHT as inhibitor

F1635

SAFC

Formaldehyde solution, contains 10-15% methanol as stabilizer, 37 wt. % in H₂O

676780

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

179124

Sigma-Aldrich

Acetone, ACS reagent, ≥99.5%

179973

Sigma-Aldrich

Acetone, Laboratory Reagent, ≥99.5%

676845

Sigma-Aldrich

Diethyl ether, ACS reagent, ≥98.0%, contains ≤2% ethanol and ≤10ppm BHT as inhibitor

32211

Sigma-Aldrich

Chloroform, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)

33120

Sigma-Aldrich

N,N-Dimethylformamide, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.8% (GC)

494488

Sigma-Aldrich

N,N-Dimethylformamide, biotech. grade, ≥99.9%

320390

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

360589

Sigma-Aldrich

Tetrahydrofuran, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%

24229

Sigma-Aldrich

Methanol, puriss., meets analytical specification of Ph Eur, ≥99.7% (GC)

24004

Sigma-Aldrich

Diethyl ether, puriss., contains ~5 mg/L 2,6-di-tert.-butyl-4-methylphenol as stabilizer, meets analytical specification of Ph. Eur., BP, ≥99.5% (GC)

676764

Sigma-Aldrich

Tetrahydrofuran, ACS reagent, ≥99.0%, contains 250 ppm BHT as inhibitor

534064

Sigma-Aldrich

Acetone, histological grade, ≥99.5%

494437

Sigma-Aldrich

Methanol, BioReagent, ≥99.93%

32201

Sigma-Aldrich

Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)