Antagonistic and synergistic effects of light irradiation on the effects of copper on Chlamydomonas reinhardtii.

Antagonistic and synergistic effects of light irradiation on the effects of copper on Chlamydomonas reinhardtii.

Aquatic toxicology (Amsterdam, Netherlands) (2014-07-30)

Giulia Cheloni, Claudia Cosio, Vera I Slaveykova

ABSTRACT

The present study showed the important role of light intensity and spectral composition on Cu uptake and effects on green alga Chlamydomonas reinhardtii. High-intenisty light (HL) increased cellular Cu concentrations, but mitigated the Cu-induced decrease in chlorophyll fluorescence, oxidative stress and lipid peroxidation at high Cu concentrations, indicating that Cu and HL interact in an antagonistic manner. HL up-regulated the transcription of genes involved in the antioxidant response in C. reinhardtii and thus reduced the oxidative stress upon exposure to Cu and HL. Combined exposure to Cu and UVBR resulted in an increase of cellular Cu contents and caused severe oxidative damage to the cells. The observed effects were higher than the sum of the effects corresponding to exposure to UVBR or Cu alone suggesting a synergistic interaction.

MATERIALS

Product Number

Brand

Product Description

12816

Sigma-Aldrich

Copper, foil, ≥99.8% (complexometric)

BCR058

Continuous cast copper (O), BCR^®, certified reference material, rod

GF74488900

Copper, tube, 200mm, outside diameter 5.0mm, inside diameter 4mm, wall thickness 0.5mm, as drawn, 99.9%

GF54770373

Copper, tube, 200mm, outside diameter 6.0mm, inside diameter 4mm, wall thickness 1.0mm, as drawn, 99.9%

GF41636921

Copper, tube, 100mm, outside diameter 2.0mm, inside diameter 1.6mm, wall thickness 0.2mm, as drawn, 99.9%

GF84156262

Copper, tube, 500mm, outside diameter 1.75mm, inside diameter 0.33mm, wall thickness 0.71mm, as drawn, 99.9%

GF84372521

Copper, tube, 200mm, outside diameter 12.7mm, inside diameter 10.26mm, wall thickness 1.22mm, as drawn, 99.9%

GF05237209

Copper, tube, 500mm, outside diameter 0.25mm, inside diameter 0.05mm, wall thickness 0.1mm, as drawn, 99.9%

GF23267976

Copper, tube, 500mm, outside diameter 10.4mm, inside diameter 10mm, wall thickness 0.2mm, as drawn, 99.9%

GF03625051

Copper - O.F.H.C., foil, 25mm disks, thickness 0.075mm, half hard, 99.95+%

GF22218847

Copper, tube, 500mm, outside diameter 0.65mm, inside diameter 0.21mm, wall thickness 0.22mm, as drawn, 99.9%

GF04615860

Copper, tube, 200mm, outside diameter 2.0mm, inside diameter 1.6mm, wall thickness 0.2mm, as drawn, 99.9%

GF08771766

Copper - O.F.H.C., foil, 1m coil, thickness 0.1mm, half hard, 99.95+%

GF10425169

Copper - O.F.H.C., foil, 20m coil, thickness 0.010mm, coil width 25mm, hard, 99.95+%

GF68687038

Copper - O.F.H.C., foil, 10m coil, thickness 0.1mm, hard, 99.95+%

GF25261657

Copper - O.F.H.C., foil, 1m coil, thickness 0.05mm, coil width 10mm, annealed, 99.95+%

GF53132330

Copper - O.F.H.C., foil, 50mm disks, thickness 0.075mm, half hard, 99.95+%

GF82329172

Copper, tube, 200mm, outside diameter 0.95mm, inside diameter 0.29mm, wall thickness 0.33mm, as drawn, 99.9%

GF58516285

Copper - O.F.H.C., foil, 20m coil, thickness 0.05mm, coil width 10mm, annealed, 99.95+%

GF19723136

Copper - O.F.H.C., foil, 25mm disks, thickness 0.125mm, hard, 99.95+%

GF69818855

Copper - O.F.H.C., foil, 50mm disks, thickness 0.05mm, hard, 99.95+%

GF20553656

Copper - O.F.H.C., foil, 5m coil, thickness 0.075mm, half hard, 99.95+%

GF26383349

Copper - O.F.H.C., foil, 5m coil, thickness 0.125mm, hard, 99.95+%

GF53533201

Copper - O.F.H.C., foil, 6mm disks, thickness 0.006mm, hard, 99.95+%

GF63169094

Copper - O.F.H.C., foil, 6mm disks, thickness 0.075mm, half hard, 99.95+%

61139

Sigma-Aldrich

Copper, turnings, purum p.a., ≥99.0%

GF87158650

Copper, tube, 500mm, outside diameter 1.0mm, inside diameter 0.24mm, wall thickness 0.38mm, as drawn, 99.9%

GF12919518

Copper - O.F.H.C., foil, 1m coil, thickness 0.1mm, hard, 99.95+%

GF00073909

Copper - O.F.H.C., foil, 4mm disks, thickness 0.006mm, hard, 99.95+%

GF46341783

Copper - O.F.H.C., foil, 2m coil, thickness 0.05mm, annealed, 99.95+%