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  • Effects of salt, sodium pyrophosphate, and sodium lactate on the probability of growth of Escherichia coli O157:H7 in ground beef.

Effects of salt, sodium pyrophosphate, and sodium lactate on the probability of growth of Escherichia coli O157:H7 in ground beef.

Journal of food protection (2011-04-12)
Cheng-An Hwang, Vijay Juneja
ABSTRACT

Ground beef products are susceptible to contamination with Escherichia coli O157:H7. The objective of this study was to examine the effect of salt, sodium pyrophosphate (SPP), and sodium lactate on the probability of growth of E. coli O157:H7 in ground beef under a temperature abuse condition. Ground beef containing 0 to 2.25% salt, 0 to 0.5% SPP, and 0 to 3% lactate was inoculated with a four-strain mixture of E. coli O157:H7, vacuum packaged, and stored at 10°C for 15 days. A total of 25 combinations of the three additives, each with 20 samples, were tested. A logistic regression was used to model the probability of growth of E. coli O157:H7 (with a 1.0-log CFU/g increase during storage) as a function of salt, SPP, and lactate. The resultant probability model indicated that lactate at higher concentrations decreased the probability of growth of E. coli O157:H7 in ground beef, and the effect was more pronounced at higher salt concentrations. At salt concentrations below 1.3%, the increase of SPP concentration marginally increased the growth probabilities of E. coli O157:H7. The model illustrated the effect of salt, SPP, and lactate on the growth probabilities and growth or no-growth behavior of E. coli O157:H7 in ground beef and can be used to improve the microbial food safety of ground beef products.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium pyrophosphate tetrabasic decahydrate, BioUltra, ≥99.5% (T)
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EN300-1250242-100MG100 mg
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€406.40
EN300-1250242-250MG250 mg
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€562.40
EN300-1250242-500MG500 mg
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€857.50
EN300-1250242-1G1 g
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€1,087.60
EN300-1250242-2.5G2.5 g
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€2,088.60
EN300-1250242-5G5 g
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€3,068.80
EN300-1250242-10G10 g
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€4,530.00

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Sigma-Aldrich
Sodium pyrophosphate tetrabasic decahydrate, BioXtra, 99.0-103.0%
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SKUVendor SKUPack SizeAvailabilityPrice
EN300-1250242-100MG100 mg
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€406.40
EN300-1250242-250MG250 mg
Ships in 14 days - from Aldrich Partner.
€562.40
EN300-1250242-500MG500 mg
Ships in 14 days - from Aldrich Partner.
€857.50
EN300-1250242-1G1 g
Ships in 14 days - from Aldrich Partner.
€1,087.60
EN300-1250242-2.5G2.5 g
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€2,088.60
EN300-1250242-5G5 g
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€3,068.80
EN300-1250242-10G10 g
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€4,530.00

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Sigma-Aldrich
Sodium pyrophosphate tetrabasic, ≥95%
Sign Into View Organizational & Contract Pricing
SKUVendor SKUPack SizeAvailabilityPrice
EN300-1250242-100MG100 mg
Ships in 14 days - from Aldrich Partner.
€406.40
EN300-1250242-250MG250 mg
Ships in 14 days - from Aldrich Partner.
€562.40
EN300-1250242-500MG500 mg
Ships in 14 days - from Aldrich Partner.
€857.50
EN300-1250242-1G1 g
Ships in 14 days - from Aldrich Partner.
€1,087.60
EN300-1250242-2.5G2.5 g
Ships in 14 days - from Aldrich Partner.
€2,088.60
EN300-1250242-5G5 g
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€3,068.80
EN300-1250242-10G10 g
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€4,530.00

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Sigma-Aldrich
Sodium pyrophosphate decahydrate, ACS reagent, ≥99%
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SKUVendor SKUPack SizeAvailabilityPrice
EN300-1250242-100MG100 mg
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€406.40
EN300-1250242-250MG250 mg
Ships in 14 days - from Aldrich Partner.
€562.40
EN300-1250242-500MG500 mg
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€857.50
EN300-1250242-1G1 g
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€1,087.60
EN300-1250242-2.5G2.5 g
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€2,088.60
EN300-1250242-5G5 g
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€3,068.80
EN300-1250242-10G10 g
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€4,530.00

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Sigma-Aldrich
Sodium DL-lactate, ReagentPlus®, ≥99.0% (NT)
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EN300-1250242-100MG100 mg
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€406.40
EN300-1250242-250MG250 mg
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€562.40
EN300-1250242-500MG500 mg
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€857.50
EN300-1250242-1G1 g
Ships in 14 days - from Aldrich Partner.
€1,087.60
EN300-1250242-2.5G2.5 g
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€2,088.60
EN300-1250242-5G5 g
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€3,068.80
EN300-1250242-10G10 g
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€4,530.00

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Sigma-Aldrich
Sodium pyrophosphate dibasic, BioUltra, ≥99.0% (T)
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EN300-1250242-100MG100 mg
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€406.40
EN300-1250242-250MG250 mg
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€562.40
EN300-1250242-500MG500 mg
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€857.50
EN300-1250242-1G1 g
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€1,087.60
EN300-1250242-2.5G2.5 g
Ships in 14 days - from Aldrich Partner.
€2,088.60
EN300-1250242-5G5 g
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€3,068.80
EN300-1250242-10G10 g
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€4,530.00

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Sigma-Aldrich
Sodium DL-lactate solution, syrup, 60 % (w/w), synthetic
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SKUVendor SKUPack SizeAvailabilityPrice
EN300-1250242-100MG100 mg
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€406.40
EN300-1250242-250MG250 mg
Ships in 14 days - from Aldrich Partner.
€562.40
EN300-1250242-500MG500 mg
Ships in 14 days - from Aldrich Partner.
€857.50
EN300-1250242-1G1 g
Ships in 14 days - from Aldrich Partner.
€1,087.60
EN300-1250242-2.5G2.5 g
Ships in 14 days - from Aldrich Partner.
€2,088.60
EN300-1250242-5G5 g
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€3,068.80
EN300-1250242-10G10 g
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€4,530.00

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Sigma-Aldrich
Sodium DL-lactate solution, synthetic, syrup, BioXtra, suitable for mouse embryo cell culture, 60 % (w/w)
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SKUVendor SKUPack SizeAvailabilityPrice
EN300-1250242-100MG100 mg
Ships in 14 days - from Aldrich Partner.
€406.40
EN300-1250242-250MG250 mg
Ships in 14 days - from Aldrich Partner.
€562.40
EN300-1250242-500MG500 mg
Ships in 14 days - from Aldrich Partner.
€857.50
EN300-1250242-1G1 g
Ships in 14 days - from Aldrich Partner.
€1,087.60
EN300-1250242-2.5G2.5 g
Ships in 14 days - from Aldrich Partner.
€2,088.60
EN300-1250242-5G5 g
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€3,068.80
EN300-1250242-10G10 g
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€4,530.00

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Sigma-Aldrich
Sodium DL-lactate solution, BioReagent, syrup, 60 % (w/w), synthetic, suitable for cell culture
Sign Into View Organizational & Contract Pricing
SKUVendor SKUPack SizeAvailabilityPrice
EN300-1250242-100MG100 mg
Ships in 14 days - from Aldrich Partner.
€406.40
EN300-1250242-250MG250 mg
Ships in 14 days - from Aldrich Partner.
€562.40
EN300-1250242-500MG500 mg
Ships in 14 days - from Aldrich Partner.
€857.50
EN300-1250242-1G1 g
Ships in 14 days - from Aldrich Partner.
€1,087.60
EN300-1250242-2.5G2.5 g
Ships in 14 days - from Aldrich Partner.
€2,088.60
EN300-1250242-5G5 g
Ships in 14 days - from Aldrich Partner.
€3,068.80
EN300-1250242-10G10 g
Ships in 14 days - from Aldrich Partner.
€4,530.00

To order products, please contact your local dealer.

Sigma-Aldrich
Sodium pyrophosphate dibasic, practical grade
Sign Into View Organizational & Contract Pricing
SKUVendor SKUPack SizeAvailabilityPrice
EN300-1250242-100MG100 mg
Ships in 14 days - from Aldrich Partner.
€406.40
EN300-1250242-250MG250 mg
Ships in 14 days - from Aldrich Partner.
€562.40
EN300-1250242-500MG500 mg
Ships in 14 days - from Aldrich Partner.
€857.50
EN300-1250242-1G1 g
Ships in 14 days - from Aldrich Partner.
€1,087.60
EN300-1250242-2.5G2.5 g
Ships in 14 days - from Aldrich Partner.
€2,088.60
EN300-1250242-5G5 g
Ships in 14 days - from Aldrich Partner.
€3,068.80
EN300-1250242-10G10 g
Ships in 14 days - from Aldrich Partner.
€4,530.00

To order products, please contact your local dealer.