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PCRH20-RO

Roche

Water, PCR Grade

liquid, pkg of 25 mL (03315959001 [1 x 25 ml]), pkg of 25 mL (03315932001 [25 x 1 ml]), pkg of 100 mL (03315843001 [4 x 25 ml])

Synonym(s):

Water, PCR Grade

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About This Item

EC Number:
231-791-2
UNSPSC Code:
12191602
NACRES:
NA.21

form

liquid

Quality Level

100

packaging

pkg of 100 mL (03315843001 [4 x 25 ml])
pkg of 25 mL (03315932001 [25 x 1 ml])
pkg of 25 mL (03315959001 [1 x 25 ml])

manufacturer/tradename

Roche

storage temp.

−20°C

General description

Water, PCR Grade is specially purified, double-distilled, deionized, and autoclaved. The production process ensures that even according to Roche ′s strict Quality Control procedures the water does not contain detectable amounts of nucleic acid. It is routinely tested in PCR with conventional thermal cyclers and with LightCycler® Instruments.

Application

This product is specially tested and qualified for use in all PCR or RT-PCR. It can be used in conjunction with all end-point or real-time PCR assays or whenever highest quality water is needed. All of the LightCycler® kits and some of the amplification products — available from Roche Applied Science — contain this Water, PCR Grade.

Quality

Function test: Each lot of Water, PCR Grade is tested in PCR (amplification of a 0.5 kb target from 0.01 ng λDNA). When the PCR product is analyzed by gel electrophoresis, a clear, defined band must be seen on the gel.
Absence of nucleases: The water is tested with various nucleic acids that could serve as nuclease substrates. No endonucleases, ribonucleases or nicking activity are detected according to the current Quality procedures.

Preparation Note

Storage conditions (working solution): Store a working solution for a short period of time (e.g. 1 week) at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

LightCycler is a registered trademark of Roche

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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We had for aim to determine the epidemiology of meningeal and lung invasive infections due to Streptococcus pneumoniae in Burkina Faso. We screened for S. pneumoniae with the usual bacteriology techniques and with real time polymerase chain reaction (rt-PCR) in 7917
Bruce Humphrey et al.
PloS one, 10(7), e0132954-e0132954 (2015-07-15)
More than two decades after its discovery, contaminant microbial DNA in PCR reagents continues to impact the sensitivity and integrity of broad-range PCR diagnostic techniques. This is particularly relevant to their use in the setting of human sepsis, where a

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