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form
suspension
Quality Level
shelf life
2 yr at −20‑0 °C
pH
~5
capacity
≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)
storage temp.
−20°C
General description
The PHOS-Select™ iron affinity gel is a novel iron [Fe(III)] chelate matrix. This matrix provides high capacity affinity binding of molecules containing phosphate groups.
Application
Phos-Select™ products facilitate a number of applications including:
- affinity enrichment of phosphopeptides obtained from protein tryptic digests
- direct transfer of phosphocompounds for analysis (HPLC or mass spectrometry)
- enrichment of phosphopeptides by immobilized metal affinity chromatography (IMAC)[1][2]
Other Notes
Phos-Select™ Iron Affinity Gel is a novel iron [Fe(III)] chelate matrix based on Sigma′s NTA analog chelate ligand. The matrix provides high capacity affinity binding of molecules containing phosphate groups.
Physical form
aqueous glycerol suspension
Legal Information
PHOS-Select is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Quantitative Phosphoproteomics of the ATM and ATR dependent DNA damage response in Arabidopsis thaliana
Roitinger E, et al.
Molecular and Cellular Proteomics, 3(10), mcp-M114 (2015)
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PloS one, 6(9), e24918-e24918 (2011-09-29)
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The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
Villen J and Gygi SP
Nature Protocols, 3(10), 1630-1630 (2008)
Jie Gao et al.
Scientific reports, 7, 40852-40852 (2017-01-21)
Low temperature is one of the key environmental stresses, which greatly affects global banana production. However, little is known about the global phosphoproteomes in Musa spp. and their regulatory roles in response to cold stress. In this study, we conducted
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Protein modifications are crucial for disease study. Analysis methods are key.
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