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  • Protonation of the oxygen axial ligand in galactose oxidase model compounds as seen with high resolution X-ray emission experiments and FEFF simulations.

Protonation of the oxygen axial ligand in galactose oxidase model compounds as seen with high resolution X-ray emission experiments and FEFF simulations.

Physical chemistry chemical physics : PCCP (2011-02-02)
Ana Mijovilovich, Sylvain Hamman, Fabrice Thomas, Frank M F de Groot, Bert M Weckhuysen
ABSTRACT

X-ray Emission Spectroscopy (XES) crossover peaks were shown to be sensitive to the protonation state of solvent molecules in the Zn protein carbonic anhydrase and its model compounds. Here we extend such studies to galactose oxidase models i.e. Cu(ii) open d-shell systems, illustrating that XES combined with FEFF8 simulations reflect changes in the protonation state of the phenolate ligand for the copper center.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Galactose Oxidase from Dactylium dendroides, lyophilized powder, ≥3,000 units/g solid
Sigma-Aldrich
Galactose Oxidase from Dactylium dendroides, ≥30 units/mg solid