MAB382
Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1
culture supernatant, clone 1, Chemicon®
Synonym(s):
Myelin A1 protein, Myelin membrane encephalitogenic protein, myelin basic protein
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
1, monoclonal
species reactivity
bovine, rat, rabbit (weakly), human
should not react with
guinea pig
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... MBP(4155)
rat ... Mbp(24547)
General description
The classic group of MBP isoforms (isoforms 4-14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Isoform 1 Golli-MBP1, HOG7, 33 kDa
Isoform 2 Golli-MBP2, HOG5, 21.5 kDa
Isoform 3 MBP1, 21.5 kDa
Isoform 4 MBP2, 20.2 kDa
Isoform 5 MBP3, 18.5 kDa
Isoform 6 MBP4, 17.2 kDa
(SP_P02686)
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Isoform 1 Golli-MBP1, HOG7, 33 kDa
Isoform 2 Golli-MBP2, HOG5, 21.5 kDa
Isoform 3 MBP1, 21.5 kDa
Isoform 4 MBP2, 20.2 kDa
Isoform 5 MBP3, 18.5 kDa
Isoform 6 MBP4, 17.2 kDa
(SP_P02686)
Specificity
Reacts with MBP from human, bovine and rat, epitope 129-138
Immunogen
Bovine myelin basic protein
Epitope: a.a. 129-138
Application
Immunohistochemistry(paraffin) Analysis:
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistology on frozen sections at 1:10
Western Blot Analysis:
A previous lot of this antibody was used in Western Blot.
ELISA:
A 1:200-1:1,000 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in Radioimmunoassay.
Optimal working dilutions must be determined by end user.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistology on frozen sections at 1:10
Western Blot Analysis:
A previous lot of this antibody was used in Western Blot.
ELISA:
A 1:200-1:1,000 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in Radioimmunoassay.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
This Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1 is validated for use in ELISA, IH, IH(P), RIA, WB for the detection of Myelin Basic Protein.
Quality
Routinely evaluated by immunohistochemistry on brain tissue.
Immunohistochemistry(paraffin) Analysis:
MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistochemistry(paraffin) Analysis:
MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Target description
19 kDa
Physical form
Culture supernatant containing 0.2 M Tris/HCl, pH 7.4 with 5-10% fetal calf serum and 0.1% sodium azide
Unpurified
Storage and Stability
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Brain tissue
Brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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