SCC453
OSUMMER.9 Mouse NRAS-Mutant Melanoma Cell Line
Mouse
Synonym(s):
Mouse melanoma cell line
About This Item
Product Name
OSUMMER.9 Mouse NRAS-Mutant Melanoma Cell Line,
biological source
mouse
Quality Level
packaging
vial of ≥1X10⁶ cells/vial vial
manufacturer/tradename
Millipore
growth mode
N/A
technique(s)
cell culture | mammalian: suitable
shipped in
liquid nitrogen
storage temp.
−196°C
1 of 4
This Item | SAB4300039 | SAB4300133 | SAB4300032 |
---|---|---|---|
rabbit | rabbit | rabbit | rabbit |
unconjugated | unconjugated | unconjugated | unconjugated |
100 | 100 | 100 | 100 |
polyclonal | polyclonal | polyclonal | polyclonal |
−20°C | −20°C | −20°C | −20°C |
Application
Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel and have been found to be negative for all pathologies in this test.
Cells are verified to be of mouse origin and negative for interspecies contamination from rat, human, Chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel.
Cells are negative for mycoplasma contamination.
Cells are genotyped by STR analysis to verify the unique identity of the cell line.
OSUMMER.6 NRAS mouse melanoma cell line was derived from a male TN mouse (homozygous for the LSL-NrasQ61R allele) exposed to UVA and UVB radiation. OSUMMER.4 can form tumors in vivo without Matrigel injection. WES (whole exome sequencing) revealed high SNV (Single Nucleotide variants) and low CNA (copy number alterations) burden, as well as the presence of UV signature mutations. OSUMMER.4 cells express the melanoma markers PMEL and MelanA and exhibit robust growth in culture. OSUMMER.6 cells demonstrate a relatively low (<lt/>200) mutation burden compared with other OSUMMER NRAS Q61R mutants, and are resistant to MEK inhibition (MEKi).
Source
OSUMMER.6 was derived from melanoma induced in TN (Tyr::CreER) mice exposed to UVA or UVB radiation on postnatal day 3 (1).
References
1. Bowman, R. L., et al. (2021). UVB mutagenesis differs in Nras-and Braf-mutant mouse models of melanoma. Life Science Alliance, 4(9).
Features and Benefits
Storage and Stability
Other Notes
Disclaimer
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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