SCC442

HPDE6c7 Human Pancreatic Duct Epithelial Cell Line

• Synonym(s): Human Pancreatic Cell Line
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human
• Growth mode: N/A
• Technique(s): cell culture | mammalian: suitable

Properties

• biological source: human
• Quality Level: 100
• packaging: vial of >1X10⁶ cells
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell culture | mammalian: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Description

Application

• Each vial contains > 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Pancreatic cancer is the fourth commonest cause of cancer death in North America and has one of the worst prognoses. Greater than 90% of these tumors arise from the pancreatic duct epithelium.1 Even when the primary tumor is small and localized, the prognosis remains poor and chemotherapy or radiotherapy has demonstrated limited effectiveness.2 The availability of dynamic models remains crucial to the study and understanding of the biological significance of the genetic changes underlying pancreatic cancer, especially in the context of pancreatic duct epithelial cell carcinogenesis.
The immortalized HPDE6c7 cell line demonstrates a near normal genotype and phenotype of pancreatic duct epithelial cells.3 HPDE6c7 cells retain normal Ki-ras, p53, C-myc, and p16INK4A genotypes but lack the p53 functional pathway.3 The HPDE6c7 cell line is useful for studies on the molecular basis of pancreatic duct cell carcinogenesis and islet cell differentiation. HPDE6c7 cells are a versatile model for the pancreatic ductal epithelium, facilitating the development of strategies for the chemoprevention of human pancreatic cancers.

Source
HPDE6c7 cells originated from normal human pancreatic duct of a 63-year-old female, having been genotypically altered by infection with retrovirus vector expressing E6E7 genes of human papilloma virus (HPV)-16.3


References
1. Am J Pathol 1996, 148(6): 1763-1770.
2. Am J Pathol 1998, 153(1): 263-269.
3. Am J Pathol 2000, 157(5): 1623-1631.

Features and Benefits

HPDE6c7 cells are a versatile model for the pancreatic ductal epithelium, facilitating the development of strategies for the chemoprevention of human pancreatic cancers.

Preparation Note

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable