MABF148E

Anti-CD161 (NK1.1) (mouse), FITC, clone PK136 Antibody

clone PK136, from mouse, FITC conjugate

• Synonym(s): Killer cell lectin-like receptor subfamily B member 1B allele A, CD161 antigen-like family member B, Lymphocyte antigen 55b (Ly-55b), NKR-P1 34, Natural killer cell surface protein NKR-P1B allele SJL/BALB (NKR-P1B), CD161b, CD161 (NK1.1)
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: PK136, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• isotype: IgG2aκ
• UniProt accession no.: P27812
• target post-translational modification: unmodified
• Gene Information: mouse ... Klrb1C(17059)

Description

General description

The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1 (Ehlers et al. 2012. Endocrinology. 10: 1247).The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1 (Carlyle et al. 2006. J. Immunol. 176: 7511-7524).

Application

This mouse monoclonal Anti-CD161 (NK1.1) (mouse), FITC, clone PK136, Cat. No. MABF1489 is validated for use in Flow Cytometry, for the detection of CD161 (NK1.1).

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD161 (NK1.1) in one million C57Bl/6 splenocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable