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Key Documents

14-755-M

Sigma-Aldrich

ULK3 Protein, active, 10 µg

Active, N-terminal 6His-tagged recombinant human full-length ULK3. For use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 62 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

General description

Product Source: N-terminal 6His-tagged recombinant human full-length ULK3. Expressed by baculovirus in Sf21 insect cells.

Biochem/physiol Actions

Protein Target: ULK3
Target Sub-Family: Misc

Quality

Routinely evaluated by phosphorylation of casein.

Physical form

50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.
Ni2+/NTA-agarose

Storage and Stability

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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