Yes, the Hemacolor reagents are suitable for use with samples anticoagulated with EDTA. If the sample is not anticoagulated, the smears must be made immediately upon the blood being drawn. These reagents are not suitable for use with samples anticoagulated with heparin or sodium citrate. Smears should be prepared from fresh samples when prepared from anticoagulated blood with EDTA.
1.11957
Hemacolor® Rapid staining of blood smear
Solution 3: colour reagent blue
About This Item
Recommended Products
Quality Level
form
liquid
IVD
for in vitro diagnostic use
technique(s)
microbe id | staining: suitable
pH
6.8 (20 °C in H2O)
density
1.01 g/cm3 at 20 °C
application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
General description
The package is sufficient for 1250 - 5000 applications. The buffer tablets acc. to Weise pH 7.2 (Product number 1.09468) are also available in larger pack sizes, thus, they can be useful for higher demands.
The three solutions needed for staining are all available individually as:
Hemacolor® Rapid staining of blood smear solution 1, fixing solution (Product number 1.11955)
Hemacolor® Rapid staining of blood smear solution 2, colour reagent red (Product number 1.11956)
Hemacolor® Rapid staining of blood smear solution 3, colour reagent blue(Product number 1.11957)
or they can be purchased as staining sets:
Hemacolor® Rapid staining of blood smear (Product number 1.11674) 3 x 100 ml
Hemacolor® Rapid staining of blood smear (Product number 1.11661) 3 x 500 ml
This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Can this kit also be used to produce blood smears where the blood was previously stored in EDTA tubes?
1 answer-
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Can products 1.11957 and 65044B-85 be used together in a procedure?
1 answer-
Products 111957-85 and 65044B-85 are each 4-liter containers of Hemacolor stain reagents. The 111957 is the blue stain reagent from set 111674, and the 65044B is the red stain reagent from set 65044-93. Upon closer examination of the stain procedures, it is revealed that there are differences in the staining procedure and how each reagent is formulated. Such a protocol is not documented. Mixing and matching reagents from different Hemacolor kits might not be as straightforward. While it is expected that the slides should stain properly, there is no documentation on file where reagents have been mixed and matched from two different kits. The procedures as published for each kit are known to work well, but whether mixing and matching reagents will yield acceptable staining is not documented. It would be safer to use the reagents listed for each kit and to follow the instructions as provided.
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