SRP6450

Neprilysin/CD10 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): CALLA, DKFZp686O16152, MGC126681, MGC126707, MME, NEP, SFE
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 80 kDa; observed mol wt 90-100 kDa (DTT-reduced. Tyr 52 is the predicted N-terminal .)
• packaging: pkg of 10 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P08473
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... MME(4311)

Description

General description

Cluster of differentiation 10 (CD10) is also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), neprilysin and common acute lymphoblastic leukemia antigen (CALLA). It is a 90-110kDa type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands and lung. The protein has a cytoplasmic amino-terminal domain, a transmembrane hydrophobic domain, and an extracellular domain which possesses catalytic activity. The gene encoding this protein is localized on human chromosome 3q25.2.

Biochem/physiol Actions

Cluster of differentiation 10 (CD10) is a zinc-dependent metalloprotease enzyme that cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. It is thought to be the rate-limiting degrading enzyme of amyloid-β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer′s disease (AD). CD10 is also identified as the common acute lymphoblastic leukemia antigen (CALLA) present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL).

Physical form

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 150 mM NaCl, pH 8.0 Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable