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SRP4675

Sigma-Aldrich

Noggin human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(s):

NOG, SYM1, SYNS1

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

~23.1 kDa

packaging

pkg of 20 μg

impurities

endotoxin, tested

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NOG(9241)

General description

Noggin (NOG) belongs to a group of diffusible proteins which bind to ligands of the TGF (transforming growth factor)-β family and regulate their activity by inhibiting their access to signaling receptors. The protein is secreted and exists as a disulfide-linked homodimer. The gene NOG is mapped to human chromosome 17q22. Recombinant human Noggin is a 23.1kDa non-disulfide-linked homodimer consisting of a total of 206 amino acid residues.

Application

Noggin (NOG) human has been used as a BMP antagonist.

Biochem/physiol Actions

Noggin (NOG) was originally identified as a BMP-4 (bone morphogenetic protein 4) antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. In mice, loss of noggin causes malformations in skeletal system. In mouse noggin is associated with tissue patterning by participating in cell differentiation, cell proliferation and apoptosis in the developing embryo. Mutations in the gene is associated with several disorders, including proximal symphalangism, multiple synostoses, tarsal/carpal coalition syndrome and Teunissen–Cremers syndrome. It is a gene which is also linked with conductive hearing loss. Polymorphism in the gene might be associated with reduced risk of nonsyndromic cleft lip with or without cleft palate (NSCLP).

Physical form

Lyophilized without any additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1 to 1.0 mg/mL. Note: Due to solubility reasons the protein should be kept at low pH. This solution can then be diluted into other aqueous buffers.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Noggin heterozygous mice: an animal model for congenital conductive hearing loss in humans.
Hwang CH and Wu DK
Human Molecular Genetics, 17, 844-853 (2008)
Association between NOGGIN and SPRY2 polymorphisms and nonsyndromic cleft lip with or without cleft palate.
Song T
American Journal of Medical Genetics. Part A, 167A, 137-141 (2015)
Nonsyndromic cleft lip with or without cleft palate: Increased burden of rare variants within Gremlin-1, a component of the bone morphogenetic protein 4 pathway.
Al Chawa T
Birth Defects Research Part A: Clinical and Molecular Teratology, 100, 493-498 (2014)
Noggin antagonism of BMP4 signaling controls development of the axial skeleton in the mouse.
Wijgerde M
Developmental Biology, 286, 149-157 (2005)
The expression patterns of gremlin 1 and noggin in normal adult and tumor tissues.
Laurila R
International Journal of Clinical and Experimental Pathology, 6, 1400-1408 (2013)

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