SRP0143

PRMT4 Active human

recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)

• Synonym(s): Arginine N-methyltransferase 4, CARM1, Coactivator-associated arginine methyltransferase 1, PRMT4
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥65% (SDS-PAGE)
• form: aqueous solution
• mol wt: 66 kDa
• packaging: pkg of 20 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_199141
• UniProt accession no.: Q86X55
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CARM1(10498)

Description

General description

Human PRMT4 (CARM1) (GenBank Accession No. NM_199141), amino acids 2-end, with N-terminal His-tag, MW = 66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.