A6029

Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Goat Anti-Human IgG (γ-chain specific)−HRP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162447
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: ELISA (d)
• Citations: 62

Properties

• biological source: goat
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): direct ELISA: 1:10,000
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat Anti-Human IgG (γ-chain specific)-Peroxidase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones Kappa and Lambda myeloma proteins.

Immunogen

purified human IgG

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Goat Anti-Human IgG (γ-chain specific)-Peroxidase antibody has been used for several ELISA-based applications.

Human serum samples were analyzed for the presence of anti-hepatitis B viral surface antigens by ELISA using HRP-conjugated goat anti-human IgG gamma chain-specific as the secondary antibody.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H315,H317,H319,H411
• pcodes: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3