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50283

Sigma-Aldrich

Anti-Mouse IgG - Atto 655 antibody produced in goat

~1 mg/mL IgG

Synonym(s):

Atto 655 [goat Anti-mouse IgG]

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

recombinant

expressed in HEK 293 cells

conjugate

Atto 655 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

contains

50% glycerol

species reactivity

mouse

concentration

~1 mg/mL IgG

technique(s)

immunofluorescence: suitable

fluorescence

λex 655 nm; λem 677 nm in PBS

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-mouse IgG (whole molecule) (Sigma, M8645) is developed in goat using purified mouse IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 655 NHS (Abs.max. 663 nm; Em.max. 684 nm),(Cat. No. 76245), then further purified via gel permeation chromatography and dialysis to remove unbound Atto-Dye.

Immunogen

purified mouse IgG

Application

Atto 655-goat anti-Mouse IgG - Atto 655 antibody has been used for immunofluorescence applications.

Packaging

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Analysis Note

Unconjugated dye ≤5% of total fluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Precautionary Statements

P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anindita Dasgupta et al.
Nature methods, 21(7), 1171-1174 (2024-06-05)
Fluorescence microscopy is limited by photoconversion due to continuous illumination, which results in not only photobleaching but also conversion of fluorescent molecules into species of different spectral properties through photoblueing. Here, we determined different fluorescence parameters of photoconverted products for
Keith Pardee et al.
Cell, 167(1), 248-259 (2016-09-24)
Synthetic biology uses living cells as molecular foundries for the biosynthesis of drugs, therapeutic proteins, and other commodities. However, the need for specialized equipment and refrigeration for production and distribution poses a challenge for the delivery of these technologies to
Nicolas Olivier et al.
Biomedical optics express, 4(6), 885-899 (2013-06-14)
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging:

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