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211-GS

Sigma-Aldrich

Endothelial Cell Growth Supplement (15ml)

Synonym(s):

Primary Cell Culture Media Supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
Pricing and availability is not currently available.

form

liquid

Quality Level

100

shelf life

1 yr

manufacturer/tradename

Cell Applications, Inc

shipped in

dry ice

storage temp.

−20°C

General description

Added to Basal Medium to create Growth Medium
Human endothelial cell growth supplement is added to the basal medium to create growth medium. The complete growth medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies.

Application

Endothelial Cell Growth Supplement (15ml) has been used as a supplement for culturing:
  • human type II alveolar cells[1]
  • human umbilical vein endothelial cells (HUVEC)[2]
  • human pulmonary artery endothelial cells (HPAECs)[3]

Quality

Each lot was tested for the ability to support plating, spreading and proliferation

Preparation Note

contained in protocol

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Certificates of Analysis (COA)

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Michelle V Mouritzen et al.
Journal of peptide science : an official publication of the European Peptide Society, 24(7), e3093-e3093 (2018-06-26)
Neurotensin, substance P, and insulin have been demonstrated to improve wound healing in vivo. However, the mechanism behind their effect is still not fully understood. This study investigates the effects leading to enhanced scratch closure by these peptides in vitro.
Koustav Ganguly et al.
Scientific reports, 10(1), 20460-20460 (2020-11-26)
Limited toxicity data on electronic cigarette (ECIG) impede evidence-based policy recommendations. We compared two popular mixed fruit flavored ECIG-liquids with and without nicotine aerosolized at 40 W (E-smoke) with respect to particle number concentrations, chemical composition, and response on physiologically relevant
Hong Xue et al.
International journal of chronic obstructive pulmonary disease, 16, 1329-1345 (2021-05-20)
Etanercept (ETN), a tumor necrosis factor-α (TNF-α) inhibitor, has been applied in the treatment of many diseases. However, whether it has effects on chronic obstructive pulmonary disease (COPD) and its interaction with tumor necrosis factor receptor 1 (TNFR1) remained unknown.

Questions

1–2 of 2 Questions  

  1. For HUVEC culture Should I add 15 ml of supplement directly to 500 ml of medium? Or Should I remove 15 ml of medium (from the original bottle) and replace this 15 ml with 15 ml of supplement?

    1 answer

    1. Complete Endothelial Cell Growth Medium is prepared by adding 15 mL of Growth Medium Supplements to 485 mL of Basal Medium.

      Helpful?

  2. Hello, could you share the concentration of this item ?

    1 answer

    1. The specific formulation for this growth supplement is considered proprietary, however, it does contain the following components:
      rhEGF
      hydrocortisone
      heparin
      FBS
      pen-strep
      amphotericin B

      Helpful?

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