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51483

Millipore

Yeast Nitrogen Base

NutriSelect® Plus, suitable for microbiology

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

product line

BioChemika

form

powder

composition

p-aminobenzoic acid, 200 μg/L
ammonium sulfate, 5 g/L
biotin, 2 μg/L
boric acid, 500 μg/L
calcium chloride, 0.1 g/L
calcium pantothenate, 400 μg/L
copper sulfate, 40 μg/L
ferric chloride, 200 μg/L
folic acid, 2 μg/L
L-histidine HCl, 10 mg/L
inositol, 2 mg/L
magnesium sulfate, 0.5 g/L
manganese sulfate, 400 μg/L
DL-methionine, 20 mg/L
niacin, 400 μg/L
potassium iodide, 100 μg/L
potassium phosphate monobasic, 1 g/L
pyridoxine HCl, 400 μg/L
riboflavin, 200 μg/L
sodium chloride, 0.1 g/L
sodium molybdate, 200 μg/L
thiamine HCl, 400 μg/L
DL-tryptophan, 20 mg/L
zinc sulfate, 400 μg/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | susceptibility testing: suitable
microbe id | utilization test: suitable
microbiological culture: suitable

final pH

5.4±0.5 (25 °C)

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical

microbiology

suitability

fungi
selective by low pH for Candida spp.
selective by low pH for Pichia spp.
selective by low pH for Saccharomyces spp.
selective by low pH for Zygosaccharomyces spp.
selective by low pH for molds (General Media)
selective by low pH for yeasts (General Media)

Application

For the classification of yeast based on carbon assimilation and for susceptibility testing of fungi.

Preparation Note

Prepare a 10x stock solution by dissolving 6.7 g and a suitable carbohydrate source in 100 ml water (warm). Filtersterilize. Prepare the final medium by dilution in sterile water.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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William A Rodríguez-Limas et al.
Microbial cell factories, 10, 33-33 (2011-05-17)
Virus-like particles (VLP) have an increasing range of applications including vaccination, drug delivery, diagnostics, gene therapy and nanotechnology. These developments require large quantities of particles that need to be obtained in efficient and economic processes. Production of VLP in yeast
Yulia Murakami et al.
Molecular and cellular biology, 28(7), 2481-2494 (2008-01-24)
Functional interactions between a mitogen-activated protein kinase (MAPK) and its regulators require specific docking interactions. Here, we investigated the mechanism by which the yeast osmoregulatory Hog1 MAPK specifically interacts with its activator, the MAPK kinase Pbs2, and its major inactivator
Influence of shaking on antifungal susceptibility testing of Cryptococcus neoformans: a comparison of the NCCLS standard M27A medium, buffered yeast nitrogen base, and RPMI?2% glucose.
Rodriguez-Tudela, Juan L., et al.
Antimicrobial Agents and Chemotherapy, 44.2, 400-404 (2000)
Ryan Rhome et al.
PloS one, 6(1), e15572-e15572 (2011-02-02)
Cryptococcus neoformans (Cn) is a significant human pathogen that, despite current treatments, continues to have a high morbidity rate especially in sub-Saharan Africa. The need for more tolerable and specific therapies has been clearly shown. In the search for novel

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Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

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