Skip to Content
Merck
  • Enhancing co-metabolic degradation of trichloroethylene with toluene using Burkholderia vietnamiensis G4 encapsulated in polyethylene glycol polymer.

Enhancing co-metabolic degradation of trichloroethylene with toluene using Burkholderia vietnamiensis G4 encapsulated in polyethylene glycol polymer.

Environmental technology (2014-04-08)
S Hamid, W Bae, S Kim, M T Amin
ABSTRACT

The biodegradation potential of Burkholderia vietnamiensis G4 (B. vietnamiensis G4) was evaluated under encapsulation in comparison with direct exposure to trichloroethylene (TCE) (0.1, 0.5, 1 and 5 mg/L) and toluene (10 and 50 mg/L), maintaining aerobic conditions. B. vietnamiensis G4 was encapsulated in polyethylene glycol (PEG) polymer. Under suspended conditions, the degradation rate decreased as the initial TCE concentration increased, even with a higher amount of substrate available. However, the encapsulated systems were less suppressed, presumably by mitigated toxicity, and completely removed TCE with 50 mg/L of toluene. The transformation yield (Ty) was as high as 0.427 mg-TCE/mg-toluene for the encapsulated cultures and 0.1007 mg-TCE/mg-toluene for the suspended cultures. The Ty value for the encapsulated cultures was one to two orders higher than what has been reported in the literature. The higher Ty values in the encapsulated cultures compared with those from suspended cultures showed that the PEG encapsulation provided more a favourable environment for efficient substrate use.

MATERIALS
Product Number
Brand
Product Description

Supelco
Toluene solution, certified reference material, 5000 μg/mL in methanol
Supelco
Trichloroethylene solution, certified reference material, 5000 μg/mL in methanol
Supelco
Trichloroethylene, analytical standard, stabilized with 30 – 50 ppm Diisopropylamine
Sigma-Aldrich
Trichloroethylene, anhydrous, contains 40 ppm diisopropylamine as stabilizer, ≥99%
Supelco
Toluene solution, certified reference material, TraceCERT®, 200 μg/mL in methanol, ampule of 1 mL
Sigma-Aldrich
Trichloroethylene, ACS reagent, ≥99.5%
Sigma-Aldrich
Toluene, anhydrous, 99.8%
Supelco
Toluene, analytical standard
Supelco
Residual Solvent - Toluene, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Residual Solvent Class 2 - Trichloroethylene, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Toluene, ACS reagent, ≥99.5%
Sigma-Aldrich
Toluene, ACS reagent, ≥99.5%
Sigma-Aldrich
Toluene, suitable for HPLC, 99.9%
Sigma-Aldrich
Toluene, ACS reagent, ≥99.5%
Sigma-Aldrich
Toluene, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.7% (GC)
Sigma-Aldrich
Toluene, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Supelco
Toluene, Pharmaceutical Secondary Standard; Certified Reference Material