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Merck
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Key Documents

93227

Millipore

Coliform ChromoSelect Agar, Modified

NutriSelect® Plus, suitable for microbiology

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

form

powder

Quality Level

200

shelf life

limited shelf life, expiry date on the label

composition

agar, 10.0 g/L
bile salts, 0.80 g/L
chromogenix mixture, 0.20 g/L
dipotassium phosphate, 0.60 g/L
magnesium sulfate, 0.20 g/L
peptone, special, 8.0 g/L
potassium dihydrogen phosphate, 0.20 g/L
sodium chloride, 1.0 g/L
yeast extract, 3.0 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

7.20±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

storage temp.

2-8°C

suitability

Escherichia coli
coliforms

Related Categories

Application

Coliform ChromoSelect Agar is recommended for the simultaneous detection of Escherichia coli and thermotolerent coliforms in water, milk, dairy products and other food samples.

Preparation Note

Suspend 24 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK5568
BCBX8496
BCBV6932
BCBQ6496

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LeMinor, L., et al.
Ann. Inst. Pasteur, 102, 267-267 (1962)
M Kilian et al.
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology, 84B(5), 245-251 (1976-10-01)
The paper describes a number of tests for the rapid detection of glycosidases including alpha-glucosidase, beta-glucosidase, beta-glucuronidase, beta-xylosidase and alpha-fucosidase. The methods use heavy suspensions of viable but non-multiplying bacteria in a buffered solution of a chromogenic substrate. The results
Frampton, E.W., et al.
Journal of Food Protection, 51, 402-402 (1988)
M Manafi et al.
Zentralblatt fur Hygiene und Umweltmedizin = International journal of hygiene and environmental medicine, 189(3), 225-234 (1989-12-01)
A comparison was made with different chromogenic and fluorogenic substrates, 4-methylumbelliferyl-beta-D-glucuronide (MUG), 4-nitrophenyl-beta-D-glucuronide (PNPG), 4-methylumbelliferyl-beta-D-galactopyranoside (MUGA), 2-nitrophenyl-beta-D-galactopyranoside (ONPG), 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-GAL), for the rapid and simultaneous enumeration of total coliforms and E. coli in water samples, based on 2 commercially available

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