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07-689

Sigma-Aldrich

Anti-EZH2 Antibody

Upstate®, from rabbit

Synonym(s):

KMT6, Enx1, Enhancer of Zeste Homologue 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

packaging

antibody small pack of 25 μL

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... EZH2(2146)

General description

Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2) is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro.

Specificity

EZH2

Immunogen

His-tagged fusion protein corresponding to residues 1-343 of mouse enhancer of Zeste homolog 2 (EZH2)

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
This Anti-EZH2 Antibody is validated for use in Immunoprecipitation and Western Blotting and ChIP-seq for the detection of EZH2.

Quality

routinely evaluated by immunoblot on RIPA lysates from HeLa and C2C12 cells

Target description

95-100kDa

Physical form

Antiserum
Format: Purified

Storage and Stability

stable for 2 years at 2-8°C from date of shipment

Analysis Note

Control
K562 cell lysate, C2C12 cell lysate

Other Notes

Please refer to lot specific datasheet.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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There is a growing interest in the pivotal role of exosomes in cancer and in their use as biomarkers. However, despite the importance of the microenvironment for cancer initiation and progression, monolayer cultures of tumor cells still represent the main
Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators.
Landeira, David, et al.
Nature Cell Biology, 12, 618-624 (2010)
Transcriptional regulation of Oct4 by a long non-coding RNA antisense to Oct4-pseudogene 5.
Hawkins, PG; Morris, KV
Transcription null
Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1.
Hong, GM; Bain, LJ
Toxicology and Applied Pharmacology null
Satoshi Kawano et al.
PloS one, 11(7), e0158888-e0158888 (2016-07-09)
The catalytic activities of covalent and ATP-dependent chromatin remodeling are central to regulating the conformational state of chromatin and the resultant transcriptional output. The enzymes that catalyze these activities are often contained within multiprotein complexes in nature. Two such multiprotein

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