Skip to Content

Fechas de fin de año: Facturación de pedidos para envío en Ciudad de México, zona Metropolitana del Valle de México y Toluca hasta 13/12. Para otras regiones, hasta el 11/12. Receso de Merck Mexico del 18/12 al 02/01.

Merck
  • Phorbol-12-myristate 13-acetate inhibits Nephronectin gene expression via Protein kinase C alpha and c-Jun/c-Fos transcription factors.

Phorbol-12-myristate 13-acetate inhibits Nephronectin gene expression via Protein kinase C alpha and c-Jun/c-Fos transcription factors.

Scientific reports (2021-10-15)
Mitsuhiro Kinoshita, Atsushi Yamada, Kiyohito Sasa, Kaori Ikezaki, Tatsuo Shirota, Ryutaro Kamijo
ABSTRACT

Nephronectin (Npnt) is an extracellular matrix protein and ligand of integrin α8β1 known to promote differentiation of osteoblasts. A search for factors that regulate Npnt gene expression in osteoblasts revealed that phorbol 12-myristate 13-acetate (PMA), which activates protein kinase C (PKC), had a strong effect to suppress that expression. Research was then conducted to elucidate the signaling pathway responsible for regulation of Npnt gene expression by PMA in osteoblasts. Treatment of MC3T3-E1 cells with PMA suppressed cell differentiation and Npnt gene expression. Effects were noted at a low concentration of PMA, and were time- and dose-dependent. Furthermore, treatment with the PKC signal inhibitor Gö6983 inhibited down-regulation of Npnt expression, while transfection with small interfering RNA (siRNA) of PKCα, c-Jun, and c-Fos suppressed that down-regulation. The present results suggest regulation of Npnt gene expression via the PKCα and c-Jun/c-Fos pathway.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Naphthol AS-MX phosphate, powder, ≥99% (HPLC)
Pricing and availability is not currently available. Contact Technical Service
Sigma-Aldrich
Anti-Actin (20-33) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Pricing and availability is not currently available. Contact Technical Service