Skip to Content
Merck
  • Parallel Reaction Monitoring reveals structure-specific ceramide alterations in the zebrafish.

Parallel Reaction Monitoring reveals structure-specific ceramide alterations in the zebrafish.

Scientific reports (2019-12-29)
Tejia Zhang, Sunia A Trauger, Charles Vidoudez, Kim P Doane, Brock R Pluimer, Randall T Peterson
ABSTRACT

Extensive characterisations of the zebrafish genome and proteome have established a foundation for the use of the zebrafish as a model organism; however, characterisation of the zebrafish lipidome has not been as comprehensive. In an effort to expand current knowledge of the zebrafish sphingolipidome, a Parallel Reaction Monitoring (PRM)-based liquid chromatography-mass spectrometry (LC-MS) method was developed to comprehensively quantify zebrafish ceramides. Comparison between zebrafish and a human cell line demonstrated remarkable overlap in ceramide composition, but also revealed a surprising lack of most sphingadiene-containing ceramides in the zebrafish. PRM analysis of zebrafish embryogenesis identified developmental stage-specific ceramide changes based on long chain base (LCB) length. A CRISPR-Cas9-generated zebrafish model of Farber disease exhibited reduced size, early mortality, and severe ceramide accumulation where the amplitude of ceramide change depended on both acyl chain and LCB lengths. Our method adds an additional level of detail to current understanding of the zebrafish lipidome, and could aid in the elucidation of structure-function associations in the context of lipid-related diseases.

MATERIALS
Product Number
Brand
Product Description

Avanti
C24:1 Ceramide-d7 (d18:1-d7/24:1), Avanti Research - A Croda Brand 860679L-1MG860746L-1MG
Avanti
C17 Ceramide (d18:1/17:0), Avanti Research - A Croda Brand
Avanti
C18 Ceramide-d7 (d18:1-d7/18:0), Avanti Research - A Croda Brand 860677P, powder
Avanti
C15 Ceramide-d7 (d18:1-d7/15:0), Avanti Research - A Croda Brand 860681P, powder
Avanti
C24:1 Ceramide-d7 (d18:1-d7/24:1(15Z)), Avanti Research - A Croda Brand 860679P, powder
Avanti
C16 Ceramide (d18:1/16:0), Avanti Research - A Croda Brand