N0513

Nerve Growth Factor-7S from murine submaxillary gland

lyophilized powder, suitable for cell culture

• Synonym(s): NGF-7S
• CAS Number: 93928-24-6
• EC Number: 210-123-3
• MDL number: MFCD00147703
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• product name: Nerve Growth Factor-7S from murine submaxillary gland, NGF-7S, lyophilized powder, suitable for cell culture
• biological source: mouse
• Quality Level: 200
• form: lyophilized powder
• potency: 2-250 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: protein 130 kDa
• packaging: pkg of 0.1 mg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤10
• color: white
• UniProt accession no.: P01139
• storage temp.: −20°C
• Gene Information: mouse ... Ngf(18049)

Description

General description

Nerve growth factor (NGF) is a dimer of 118-residue polypeptide chains linked non-covalently. Both these polypeptides contain three intramolecular disulphide bridges. It forms the prototype of the neurotrophin growth factor family. In male mouse submandibular salivary gland, NGF is present in high amounts, and is present as a high molecular weight complex called nerve growth factor-7S (NGF-7S). NGF-7S is an α₂β₂γ₂ complex, where β₂-NGF is the active neurotrophin, and is linked to the dimers of the glandular kallikrein serine proteinases members, α-NGF and γ-NGF.

Application

Nerve Growth Factor-7S (NGF-7S) from murine submaxillary gland has been used for the treatment of following cells to induce neuronal differentiation-

• PC12
• Dorsal root ganglia (DRG) obtained from chick embryos of either sex (Gallus gallus)

Biochem/physiol Actions

Nerve growth factor (NGF) is a neurotrophin, which facilitates the differentiation and survival of specific subtypes of neurons of central nervous system and peripheral nervous system. However, nerve growth factor-7S (NGF-7S) lacks any neurotrophic activity as most part of β-NGF subunit is buried within the NGF-7S complex.

Physical form

Lyophilized from 0.2 μm filtered solution in 25 mM sodium phosphate, pH 7.0.

Preparation Note

Nerve growth factor-7S (NGF-7S) is isolated from male mouse submaxillary glands using a modification of the method of Varon, et al.

Analysis Note

The biological activity is measured using a 3-day MTT assay.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves