H8264

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): HBSS
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: phenol red: no; glucose: 1.0 g/L (Dextro); NaHCO₃: 0.35 g/L
• shipped in: ambient

Description

General description

Hanks’ Balanced Salt solution (HBSS) is a buffer that mimics biological medium and stabilizes cells. It is useful in leucocyte labeling studies. HBSS is an effective preservative for periodontal ligament cells. HBSS lacks essential inorganic ions and is useful in starvation studies.

Application

Calcium and magnesium are included in this formulation and support cell adhesion. Used to minimize cell attachment or aggregation.


Hanks’ Balanced Salt solution has been used:

• as a rinse solution for hepatocytes
• for hippocampi dissection
• in corrosion testing of dog-bone specimens
• for starvation studies in breast cancer cell line
• as a component of dissociation solution for dorsal root ganglion
• as a chamber solution for the patch-clamp apparatus

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable