- Nanobioconjugates of Candida antarctica lipase B and single-walled carbon nanotubes in biodiesel production.
Nanobioconjugates of Candida antarctica lipase B and single-walled carbon nanotubes in biodiesel production.
Bioresource technology (2015-11-23)
László Csaba Bencze, Judith H Bartha-Vári, Gabriel Katona, Monica Ioana Toşa, Csaba Paizs, Florin-Dan Irimie
PMID26590760
ABSTRACT
Carboxylated single-walled carbon nanotubes (SWCNTCOOH) were used as support for covalent immobilization of Candida antarctica lipase B (CaL-B) using linkers with different lengths. The obtained nanostructured biocatalysts with low diffusional limitation were tested in batch mode in the ethanolysis of the sunflower oil. SWCNTCOOH-CaL-B proved to be a highly efficient and stable biocatalyst in acetonitrile (83.4% conversion after 4h at 35°C, retaining >90% of original activity after 10 cycles).
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Chloroform-d, "100%", 99.96 atom % D, contains 0.5 wt. % silver wire as stabilizer
Sigma-Aldrich
Chloroform-d, ≥99.8 atom % D, contains 0.5 wt. % silver foil as stabilizer, 0.03 % (v/v) TMS
Sigma-Aldrich
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.