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  • ELISA detection of semicarbazide based on a fast sample pretreatment method.

ELISA detection of semicarbazide based on a fast sample pretreatment method.

Chemical communications (Cambridge, England) (2013-06-04)
Zhiyuan Fang, Baishan Jiang, Wei Wu, Zhicheng Xiang, Chuanyan Ouyang, Tulong Huang, Junhua Chen, Lingwen Zeng
PMID23728222
ABSTRACT

A direct ELISA was established for the fast detection of semicarbazide (SEM) using a novel biotin derivative. Without a tedious extraction procedure, as low as 0.07 μg L(-1) of SEM could be detected reproducibly. This assay has better recovery and accuracy than competitive ELISA.