Technically, there is no limit to the percentage of water that can be used on a bare silica column. However, exposing the column to 100% water would require several hours to re-equilibrate back to typical HILIC conditions. Nevertheless, it is advisable to use 95% water and 5% Acetonitrile to avoid dewetting the phase, unless the column is explicitly labeled as aqueous compatible. After flushing the column sufficiently, a re-equilibration of no fewer than 75 column volumes is recommended.
581502-U
Ascentis® Si (3 µm) HPLC Columns
L × I.D. 15 cm × 2.1 mm, HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
€918.00
Please contact Customer Service for Availability
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Product Name
Ascentis® Si HPLC Column, 3 μm particle size, L × I.D. 15 cm × 2.1 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L3
product line
Ascentis®
feature
endcapped: no
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
15 cm × 2.1 mm
surface area
450 m2/g
impurities
<5 ppm metals
matrix
fully porous particle
silica gel high purity, spherical
matrix active group
silica phase
particle size
3 μm
pore size
100 Å
operating pH range
2-6
application(s)
food and beverages
separation technique
hydrophilic interaction (HILIC)
normal phase
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General description
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.
The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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Deborah Benhaim et al.
Journal of chromatography. A, 1217(1), 65-74 (2009-11-27)
This study investigates lipophilicity determination by chromatographic measurements using the polar embedded Ascentis RP-Amide stationary phase. As a new generation of amide-functionalized silica stationary phase, the Ascentis RP-Amide column is evaluated as a possible substitution to the n-octanol/water partitioning system
Paweł Kubica et al.
Journal of chromatography. A, 1289, 13-18 (2013-04-04)
Two analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach
Shigehisa Uchiyama et al.
Analytical chemistry, 80(9), 3285-3290 (2008-03-18)
A new method for the simultaneous determination of ozone and carbonyls in air using a two-bed cartridge system has been developed. Each bed consists of reagent-impregnated silica particles. The first contains trans-1,2-bis-(4-pyridyl) ethylene (BPE) while the second contains 2,4-dinitrophenylhydrazine (DNPH).
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 50(5), 703-709 (2009-06-30)
The performance of a narrow bore silica based monolith column (5 cm x 2 mm) was compared to 5 cm long narrow bore (internal diameter < or = 2.1 mm) columns, packed with shell particles (2.7 microm) and totally porous
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 49(1), 64-71 (2008-11-29)
The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 microm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities
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What is the maximum percentage of water that should not be exceeded when using this column to prevent deactivation of the silica?
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