M8064

Anti-Myosin IIA, non muscle antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-BDPLT6, Anti-DFNA17, Anti-EPSTS, Anti-FTNS, Anti-MATINS, Anti-MHA, Anti-NMHC-II-A, Anti-NMMHC-IIA, Anti-NMMHCA
• MDL number: MFCD03455610
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~200 kDa
• species reactivity: rat, human, canine
• packaging: antibody small pack of 25 μL
• technique(s): indirect immunofluorescence: 1:100 using cultured rat NRK cells; microarray: suitable; western blot: 1:1,000 using whole cell extracts of cultured dog MDCK kidney cells and cultured human Jurkat cells
• UniProt accession no.: P35579
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MYH9(4627) ; rat ... Myh9(25745)

Description

General description

Myosin heavy chain 9 (MYH9) gene codes for the nonmuscle myosin heavy chain IIA.

Myosin heavy chain 9 is expressed in platelets and the gene encoding it is localized on chromosome 22.

Immunogen

synthetic peptide corresponding to amino acids 1949-1960 of human nonmuscle myosin IIA.

Application

Anti-Myosin IIA, non muscle antibody produced in rabbit has been used in:

• immunofluorescence
• immunohistochemistry
• fixed cell staining/ immunofluorescence staining
• immunoblot
• immunoprecipitation

Biochem/physiol Actions

Nonmuscle myosin II is involved in cell motility and adhesion, cytokinesis, vesicular transport, intracellular force generation and in morphogenesis during development. Its activity is regulated by light chain and possibly heavy chain phosphorylation and by association with proteins such as Mts1. Mutations in the NMHCA gene are found in several syndromes associated with megakaryocyte/platelet/leukocyte disorders. Mutations in the MYH9 gene causes a spectrum of macrothrombocytopenia disorders with neutrophil inclusions, termed as MYH9 disorders.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable