1.04044

GranuCult^® ENDO agar

for Escherichia coli, for coliforms, suitable for microbiology

• Synonym(s): Coliform selective medium, Endo C agar, Fuchsin lactose agar acc. to ENDO
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: according to ENDO
• Quality Level: 200
• sterility: non-sterile
• form: granular
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• technique(s): microbiological culture: suitable
• color: red-violet
• pH: 7.2-7.6 (37 °C, 39 g/L in H₂O, after autoclaving)
• solubility: 39 g/L
• bulk density: 700 kg/m³
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Escherichia coli; coliforms

Description

General description

GranuCult^® ENDO agar is a selective medium recommended for the isolation of Escherichia coli and coliform bacteria. Sodium sulfite and fuchsin inhibit the growth of gram-positive bacteria. E. coli and coliform bacteria metabolize lactose with the production of aldehyde and acid. The aldehyde liberates fuchsin from the fuchsin-sulfite compound, the fuchsin then colors the colonies red. In the case of E. coli, this reaction is so intense that the fuchsin crystallizes, giving the colonies a permanent greenish metallic sheen (fuchsin sheen). Lactose-negative and weakly lactose-positive E. coli do not show any fuchsin sheen.

Application

ENDO agar is suitable for the detection and isolation of E. coli and coliform bacteria in various materials.

Features and Benefits

• GranuCult^® offers superior granulated culture media.
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions.
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Storage and Stability

Keep tightly closed. Keep in a well-ventilated place. Keep locked up or in an area accessible only to qualified or authorized person.

Analysis Note

Appearance (clearness): clear to slightly turbid
Appearance (colour): pink/red
pH-value (25 °C): 7.2 - 7.6
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli 194): good to very good
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good to very good
Growth (Enterobacter cloacae ATCC 13047 (WDCM 00083)): good to very good
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Shigella flexneri ATCC 12022 (WDCM 00126)): good to very good
Growth (Proteus mirabilis ATCC 14153): good to very good
Growth (Enterococcus faecalis ATCC 11700): none to fair
Red colonies (Escherichia coli ATCC 25922 (WDCM 00013)): +
Red colonies (Escherichia coli 194): +
Red colonies (Escherichia coli ATCC 11775 (WDCM 00090)): +
Red colonies (Enterobacter cloacae ATCC 13047 (WDCM 00083)): +(poor)
Red colonies (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Red colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Red colonies (Shigella flexneri ATCC 12022 (WDCM 00126)): -
Red colonies (Proteus mirabilis ATCC 14153): -
Metallic lustre (Escherichia coli ATCC 25922 (WDCM 00013)): +
Metallic lustre (Escherichia coli 194): +
Metallic lustre (Escherichia coli ATCC 11775 (WDCM 00090)): +(poor)
Metallic lustre (Enterobacter cloacae ATCC 13047 (WDCM 00083)): - or poor
Metallic lustre (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): -
Metallic lustre (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Metallic lustre (Shigella flexneri ATCC 12022 (WDCM 00126)): -
Metallic lustre (Proteus mirabilis ATCC 14153): -
Incubation: 24 hrs. 35 °C

Other Notes

• The plates are clear and pale pink.
• If the culture medium is somewhat red after it has solidified, the red coloration can be removed by adding a few drops (max. 1 mL/liter) of a freshly prepared 10 % sodium sulfite solution and then boiling.
• On exposure to oxygen, the plated culture medium gradually becomes red due to the oxidation of sulfite and can thus no longer be used. It can only be kept for a few days even if it is stored in the dark and at refrigerator temperature.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H350
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3