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  • Asymmetric synthesis of (S)-alpha-methylbenzylamine by recombinant Escherichia coli co-expressing omega-transaminase and acetolactate synthase.

Asymmetric synthesis of (S)-alpha-methylbenzylamine by recombinant Escherichia coli co-expressing omega-transaminase and acetolactate synthase.

Bioscience, biotechnology, and biochemistry (2008-11-11)
Hyungdon Yun, Byung-Gee Kim
PMID18997410
ABSTRACT

To produce (S)-alpha-methylbenzylamine (MBA) from acetophenone, recombinant Escherichia coli co-expressing omega-transaminase and acetolactate synthase was used as a whole-cell biocatalyst. The solvent-bridge reaction system increased the yield of the whole-cell reaction by 2.5-fold, and the inhibitory (S)-alpha-MBA produced in the omega-transaminase reaction solution (pH 8.0) moved into the extraction solution (pH 3.0) via an organic solvent.

MATERIALS
Product Number
Brand
Product Description
M31104
Sigma-Aldrich
α-Methylbenzylamine, 99%
115541
Sigma-Aldrich
(R)-(+)-α-Methylbenzylamine, 98%
77879
Supelco
(R)-(+)-α-Methylbenzylamine, for chiral derivatization, LiChropur™, ≥99.0%
77880
Sigma-Aldrich
(R)-(+)-α-Methylbenzylamine, purum, ≥98.0% (sum of enantiomers, GC)
726621
Sigma-Aldrich
(R)-(+)-α-Methylbenzylamine, ChiPros®, produced by BASF, ≥99.0%
115568
Sigma-Aldrich
(S)-(−)-α-Methylbenzylamine, 98%