Fluorescence in situ hybridization in plants: recent developments and future applications.

Fluorescence in situ hybridization in plants: recent developments and future applications.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology (2019-03-11)

Jiming Jiang

PMID30852707

ABSTRACT

Fluorescence in situ hybridization (FISH) was developed more than 30 years ago and has been the most paradigm-changing technique in cytogenetic research. FISH has been used to answer questions related to structure, mutation, and evolution of not only individual chromosomes but also entire genomes. FISH has served as an important tool for chromosome identification in many plant species. This review intends to summarize and discuss key technical development and applications of FISH in plants since 2006. The most significant recent advance of FISH is the development and application of probes based on synthetic oligonucleotides (oligos). Oligos specific to a repetitive DNA sequence, to a specific chromosomal region, or to an entire chromosome can be computationally identified, synthesized in parallel, and fluorescently labeled. Oligo probes designed from conserved DNA sequences from one species can be used among genetically related species, allowing comparative cytogenetic mapping of these species. The advances with synthetic oligo probes will significantly expand the applications of FISH especially in non-model plant species. Recent achievements and future applications of FISH and oligo-FISH are discussed.

MATERIALS

Product Number

Brand

Product Description

MBD0040

Sigma-Aldrich

Proteus FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0029

Sigma-Aldrich

Porphyromonas gingivalis FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0032

Sigma-Aldrich

Eubacteria FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH), 20 µM in water

MBD0049

Sigma-Aldrich

Escherichia coli FISH probe -ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water

MBD0035

Sigma-Aldrich

Bacterial negative control FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0047

Sigma-Aldrich

Enterococcus faecium FISH probe – ATTO 488, Probe for fluorescence in situ hybridization (FISH), 20µM in water

MBD0048

Sigma-Aldrich

Enterococcus faecium FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH), 20µM in water

MBD0050

Sigma-Aldrich

Akkermansia muciniphila FISH probe-ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water

MBD0041

Sigma-Aldrich

Akkermansia muciniphila FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0059

Sigma-Aldrich

Alphaproteobacteria FISH probe-Cy3, Probe for fluorescence in situ hybridization (FISH), 20 μM in water

MBD0042

Sigma-Aldrich

Escherichia coli FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH)

MBD0033

Sigma-Aldrich

Eubacteria FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH)

MBD0028

Sigma-Aldrich

Porphyromonas gingivalis FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH)

MBD0034

Sigma-Aldrich

Bacterial negative control FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0039

Sigma-Aldrich

Proteus FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH),20 μM in water