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G006123

HPLC Analysis of Morphine and Glucuronide Metabolites on Discovery® HS F5

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery HS F5, 5 cm x 4.6 mm I.D., 5 μm particles (567513-U)
mobile phase: [A] 10 mM ammonium acetate, pH 6.8 (unadjusted); [B] acetonitrile
gradient: 0% B for 1 min, to 30% B in 4 min, held 3 min
flow rate: 1 mL/min
column temp.: 40 °C
detector: UV, 280 nm
injection: 10 μL
sample: 50 μg/mL in final mobile phase

suitability

application for HPLC

application(s)

clinical
pharmaceutical (small molecule)

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Morphine 3-ß-D-glucuronide
    Morphine-6-β-D-glucuronide solution, 100 μg/mL in methanol: water (1:1), ampule of 1 mL, certified reference material, Cerilliant®
    Morphine solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

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