808647
TSKgel® Chelate-5PW (20 µm) HPLC Columns
L x I.D. 2.5 cm × 6 mm, iminodiacetic acid phase
Synonym(s):
TSKgel® Chelate-5PW HPLC Column and Guardgel Kit
About This Item
product name
TSKgel® Chelate-5PW Guardgel Kit, phase iminodiacetic acid, L × I.D. 2.5 cm × 6 mm, 20 μm particle size
material
PEEK
product line
TSKgel®
manufacturer/tradename
Tosoh Chelate-5PW, 08647
extent of labeling
20 μmol/mL resin ligand density
parameter
≤20% organic solvent
≤3 M salt concentration
≤45 °C temperature
technique(s)
LPLC: suitable
L × I.D.
2.5 cm × 6 mm
matrix
polymer particle platform
matrix active group
iminodiacetic acid phase
particle size
20 μm
operating pH
2-12
compatibility
mode of use affinity HPLC
separation technique
affinity
General description
TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.
Components
- 5 mL Guardgel packing material
- Column holder, consisting of external reducing union, cap, frit, 2 ferrules
- Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 μm frit at 10 mm depth, 1/16"
- 2 Replacement frits
- Capillary tubing, 5 cm x 1/16" OD x 0.01"
- Nuts and ferrules, 10-32 threads for 1/16" tubing, 2 each
- All hardware is 316 stainless steel
Dimensions for Guardgel kits for 21.5 mm ID columns:
- Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 μm frit at 20mm depth, 1/16"
- Capillary tubing, 5 cm x 1/16" OD x 0.02" ID
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