The principal of this kit is based on chemical binding and elution rather than size exclusion. To recover more of the small-sized RNA, an increase the amount of Binding Solution is suggested, however some small RNA molecules, including microRNA, siRNA, tRNA, and 5S rRNA, are not efficiently recovered. To maximize the recovery of small RNAs, refer to Protocol A on page 5 of the user guide – link provided below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/136/spectrumrnakitug-ms.pdf
STRN50
Spectrum™ Plant Total RNA Kit
Greener Alternative
sufficient for 50 purifications
Synonym(s):
Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Available to ship onJuly 07, 2026fromMILWAUKEE | $392.00 |
About This Item
NACRES:
NA.52
UNSPSC Code:
41105501
usage
sufficient for 50 purifications
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
RNA purification: suitable
test parameters
: ~30 min kit run time, sample volume: 100 mg
greener alternative category
storage temp.
15-25°C
General description
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.
Application
Spectrum Plant Total RNA Kit has been used to isolate RNA from ground fiber tissue of cultured cotton fiber, from neem tissues and from Sarracenia, an insectivorous plant.[1]
Features and Benefits
- Yields up to 60 μg of pure concentrated RNA per prep
- Efficient protocol allows for RNA purification in 30 min or less
- Specially designed for research with difficult plant tissues
Legal Information
Spectrum is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable |
| usage sufficient for 50 purifications | usage sufficient for 250 purifications | usage sufficient for 350 purifications | usage sufficient for 10 purifications |
| test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters : <30 min kit run time, sample volume: up to 10^7 cells or 40 mg of tissue | test parameters : ~30 min kit run time, sample volume: 100 mg |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| greener alternative category | greener alternative category | greener alternative category | greener alternative category , Aligned |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
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Kit Components Only
Product No.
Description
- Binding Solution
- Lysis Solution
- Wash Solution 1
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
- 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
signalword
Danger
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
target_organs
Liver,Heart
wgk
WGK 3
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
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Which is the exclusion size for RNAs purified with this kit ? I am wondering in the retention size of small RNAs
1 answer-
Helpful?
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What composition of your elution buffer (E8024-50ml) in plant total RNA kit?
1 answer-
The composition of this product is considered proprietary.
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Is it suitable for use with chestnut samples?
Is it suitable for use with chestnut samples?
1 answer-
The kit has been utilized for chestnut samples, as reported in the following paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615883/
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We need to know the elution buffer composition for downstream quantification and sequencing. What solution can be used besides the original elution buffer that can have the same proprerties to be used as a blank?
1 answer-
A suitable solution would be molecular biology-grade water or nuclease-free water. These types of water are typically free of contaminants and nucleases, making them ideal for use as a blank solution in downstream quantification and sequencing experiments.
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