SRP5148
Tubulin, GST tagged human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB5
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About This Item
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biological source
human
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
~76 kDa
NCBI accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... TUBB(203068)
General description
Tubulin-β 1 (TUBB1) protein is a major constituent of microtubules. Previous work suggested that the "microtubule binding domain" of tau (composed of three or four imperfect 18-amino acid repeats, separated by 13- or 14-amino acid inter-repeat regions) can bind to the C-terminal ends of both α and β tubulin monomers. It is found in hematopoietic cells and platelet marginal bands. The gene encoding TUBB1 is localized on human chromosome 6p21.
Application
GST tagged tubulin has been used in Western blotting and in mass spec as a control.
Biochem/physiol Actions
Tubulin-β 1 (TUBB1) interaction with microtubule-associated proteins (MAPs) such as tau is fundamental for microtubule structure and function. It has a role in the formation of the cytoskeleton. Studies revealed that TUBB1 is the target of various antitubulin agents used in the treatment of cancer. Subsequent studies have also concluded that TUBB1 mutations in clinical samples are rare, and unlikely to contribute to drug resistance.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The beta 1 tubulin R307H single nucleotide polymorphism is associated with treatment failures in immune thrombocytopenia (ITP)
Paula A
British Journal of Haematology (2013)
An expressed beta-tubulin gene, TUBB, is located on the short arm of human chromosome 6 and two related sequences are dispersed on chromosomes 8 and 13.
Floyd_smith G
Experimental Cell Research (1986)
M F Chau et al.
Biochemistry, 37(51), 17692-17703 (1999-01-28)
The interaction between tubulin subunits and microtubule-associated proteins (MAPs) such as tau is fundamental for microtubule structure and function. Previous work has suggested that the "microtubule binding domain" of tau (composed of three or four imperfect 18-amino acid repeats, separated
Helen K Berrieman et al.
The Lancet. Oncology, 5(3), 158-164 (2004-03-09)
beta-tubulin is the target of various antitubulin agents used in the treatment of cancer. After beta tubulin was shown to confer resistance to antitubulin agents in established cell lines, several studies have investigated the DNA sequence of this compound in
The intricate relationship between microtubules and their associated motor proteins during axon growth and maintenance
Andreas Prokop
Neural Dev. (2013)
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