SAE0156
Bone Alkaline Phosphatase from human bone osteosarcoma cells
lyophilized, ≥2000 u/mg protein
Synonym(s):
ALPL, BAP, Bone ALP, Ostase
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| Size/SKU | Availability | Price |
|---|---|---|
5 μg | Check Cart for Availability | $649.00 |
About This Item
NACRES:
NA.77
UNSPSC Code:
12352204
$649.00
Check Cart for Availability
General description
Mammalian alkaline phosphatases are a group of membrane-bound glycoprotein isoenzymes that are attached to the cell membrane by a hydrophobic glycosyl-phosphatidylinositol (GPI) anchor.
The bone alkaline phosphatase isozyme is expressed from the gene for tissue nonspecific alkaline phosphatase which is also expressed in liver and kidney. These three isoforms form the majority of alkaline phosphatases circulating in serum. All three forms share the same amino acid sequence but have different carbohydrate and lipid modifications that provide unique properties according to the source tissue.
Bone alkaline phosphatase is synthesized by osteoblasts, involved in the calcification of bone matrix, but its precise role in bone formation process is still unknown. It is a highly specific marker of the bone-forming activity of osteoblasts. High activity of bone alkaline phosphatase is observed in serum in several bone diseases, such as Paget′s disease, osteoporosis, ricket disease, bone metastatic carcinoma, and others. Physiological bone growth can also contribute to increased levels of bone alkaline phosphatase in serum.
This product is purified from human osteosarcoma Saos-2 cells, an established cell line with high basal alkaline phosphatase activity. Before purification, alkaline phosphatase is released from the cell membrane by enzymatic cleavage of the GPI anchor.
The bone alkaline phosphatase isozyme is expressed from the gene for tissue nonspecific alkaline phosphatase which is also expressed in liver and kidney. These three isoforms form the majority of alkaline phosphatases circulating in serum. All three forms share the same amino acid sequence but have different carbohydrate and lipid modifications that provide unique properties according to the source tissue.
Bone alkaline phosphatase is synthesized by osteoblasts, involved in the calcification of bone matrix, but its precise role in bone formation process is still unknown. It is a highly specific marker of the bone-forming activity of osteoblasts. High activity of bone alkaline phosphatase is observed in serum in several bone diseases, such as Paget′s disease, osteoporosis, ricket disease, bone metastatic carcinoma, and others. Physiological bone growth can also contribute to increased levels of bone alkaline phosphatase in serum.
This product is purified from human osteosarcoma Saos-2 cells, an established cell line with high basal alkaline phosphatase activity. Before purification, alkaline phosphatase is released from the cell membrane by enzymatic cleavage of the GPI anchor.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate to p-nitrophenol and inorganic phosphate per minute at pH 9.8 at 37 °C.
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This Item | |||
|---|---|---|---|
| description lyophilized, ≥2000 u/mg protein | description sufficient for 100 tests | description - | description clone BAP-77, purified from hybridoma cell culture |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
Storage Class
11 - Combustible Solids
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