S6446

Anti-SMC1L1 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-SMC1, Anti-SMC1 structural maintenance of chromosomes 1-like 1, Anti-SMC1α
• MDL number: MFCD03791776
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 160 kDa
• species reactivity: rat (predicted), human, mouse (predicted)
• technique(s): immunoprecipitation (IP): 5-10 μL using HEK 293-T cell lysates; indirect immunofluorescence: 1:100-1:200 using paraformaldehyde-fixed HEK 293-T cells; microarray: suitable; western blot: 1:500-1:1,000 using HeLa nuclear extracts
• UniProt accession no.: Q14683
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SMC1A(8243) ; mouse ... Smc1a(24061) ; rat ... Smc1a(63996)

Description

General description

Structural maintenance of chromosomes protein 1A (SMC1A, SMC1L1, SMC1), a structural maintenance of chromosomes (SMC) protein belongs to the SMC superfamily and is an important constituent of the cohesin complex. This gene is located on human chromosome Xp11.22.

Specificity

Anti SMC1L1 (also known as SMC1 and SMC1a) specifically recognizes SMC1L1.

Immunogen

synthetic peptide corresponding to amino acids 1210-1225 of human SMC1L1, conjugated to KLH via an N-terminal cysteine residue. The immunizing sequence is conserved in mouse and rat.

Application

Anti-SMC1L1 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Structural maintenance of chromosomes protein 1A (SMC1A, SMC1L1, SMC1) can be phosphorylated by Ataxia-telangiectasia, mutated (ATM) indicating a potential role for this protein in DNA repair. Thus, SMC1L1 phosphorylation appears to be the critical downstream event in the ATM-nibrin (NBS1)-breast cancer type 1 (BRCA1) pathway, which mediates cell survival and chromosomal stabilization after DNA damage. Mutations in SMC1L1 gene result in the Cornelia de Lange syndrome, a multisystem developmental disorder. It participates in chromosome dynamics, double-strand break (DSB) repair and tumorigenesis. This gene can control the cell cycle and helps to maintain the stability of genome. Suppression of SMC1A can block the proliferation and migration of hepatocellular carcinoma cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)