S1265

Anti-Sheep IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162843
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i)
• Citations: 14

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): indirect ELISA: 1:50,000-1:100,000
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins. Antiserum is determined to be immunospecific for sheep IgG by immunoelectrophoresis (IEP) versus sheep IgG and normal sheep serum. Identity and purity of the antibody is established by immunoelectrophoresis (IEP). Electrophoresis of the product followed by diffusion versus the anti-rabbit IgG and the anti-rabbit whole serum result in single arcs of precipitation in the γ region.

Immunogen

purified sheep IgG

Application

Anti-Sheep IgG (whole molecule) antibody produced in rabbit has been used in:

• Enzyme-linked immunosorbent assay (ELISA)
• Immunoprecipitation[1]
• labelling electrodes in immunosensor systems

Biochem/physiol Actions

Binds all sheep Igs

Digestion of IgG by papain generates fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallisable (fc). IgG antibody has enormous therapeutic potential and the Fc is involved in the development of therapeutic antibody.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)