Yes, RIPA buffer would be a suitable lysis solution for use with this kit.
RAB0012
Phospho-Akt (pSer473) / pan-Akt ELISA Kit
for detection of human, mouse or rat phospho-akt (pSer473) and pan-akt in cell and tissue lysates
Synonym(s):
Akt ELISA Kit
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery) | $851.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... AKT1(207)
Input:
sample type: human cell lysate
sample type tissue lysate
sample type tissue lysate
Species reactivity:
human, mouse, rat
Storage temp.:
−20°C
Shipped in:
wet ice
$851.00
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
species reactivity
human, mouse, rat
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type: human cell lysate
sample type tissue lysate
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... AKT1(207)
General description
The Phospho-Akt (pSer473) ELISA (Enzyme-Linked Immunosorbent Assay) kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates.
Immunogen
Akt (pSer473) synthetic phosphopeptide, Recombinant Human EGFR
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
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This Item | |||
|---|---|---|---|
| description for detection of human, mouse or rat phospho-akt (pSer473) and pan-akt in cell and tissue lysates | description for detection of human, mouse or rat phospho-akt (pSer473) in cell and tissue lysates | description affinity isolated antibody | description affinity isolated antibody |
| species reactivity human, mouse, rat | species reactivity human, rat, mouse | species reactivity rat, human, mouse | species reactivity mouse, human, rat |
| Gene Information human ... AKT1(207) | Gene Information human ... AKT1(207) | Gene Information human ... AKT1(207) | Gene Information human ... AKT1(207) |
| input sample type: human cell lysate | input sample type cell lysate | input - | input - |
| technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) western blot: 1:500-1:1000 (Cell Lysate) | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100, western blot: 1:500-1:1000 |
| detection method colorimetric | detection method colorimetric | detection method - | detection method - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2
Storage Class
12 - Non Combustible Liquids
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Is this kit compatible with RIPA buffer
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