This product is prepared from material with a specific activity of 150 units per mg. Therefore there is approximately 20 micrograms per vial.
P2289
Endoproteinase Lys-C from Lysobacter enzymogenes
lyophilized powder
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
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form
lyophilized powder
Quality Level
analyte chemical class(es)
amino acids
packaging
vial of ≥3.0 units
storage temp.
2-8°C
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General description
Endoproteinase Lys-C is an enzyme that preferentially cleaves at the carboxyl side of lysine residues. [1]
Application
Endoproteinase Lys-C from Lysobacter enzymogenes is useful in the determination of primary structures of proteins. [1] It has been used in a study to investigate the evidence for trisulfide bonds in a recombinant variant of a human IgG2 monoclonal antibody. [2]
Endoproteinase Lys-C has been used for the digestion monoclonal antibodies for disulfide bond assignment. [3]
Biochem/physiol Actions
Endoproteinase Lys-C inactivates the enzyme inositol monophosphatase by cleaving it at a single site directly after Lys 36. [4]
Unit Definition
One unit will hydrolyze 1.0 μmole of N-p-tosyl-Gly-Pro-Lys p-nitroanilide per min at pH 7.7 at 25 °C.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Hiromi Ito et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 806(1), 11-17 (2004-05-20)
Formation of covalently bound protein adducts with 2-arylpropionic acids (2-APAs) has been proposed as a possible explanation for hypersensitivity and toxic responses to chiral carboxylic acid drugs. To identify the cellular proteins chemically modified with optically active (S)-ibuprofen, we generate
J G Jacobs et al.
Journal of virology, 85(23), 12537-12546 (2011-09-16)
Classical scrapie is a prion disease in sheep and goats. In sheep, susceptibility to disease is genetically influenced by single amino acid substitutions. Genetic breeding programs aimed at enrichment of arginine-171 (171R) prion protein (PrP), the so-called ARR allele, in
Wei Zhang et al.
Analytical biochemistry, 311(1), 1-9 (2002-11-21)
Recombinant monoclonal antibodies (mAbs) are an emerging therapeutic area. However, there are few reports on disulfide bond assignment of recombinant mAbs. This work describes the complete disulfide bond assignment of a recombinant immunoglobulin G4 (IgG4) mAb. N-ethylmaleimide (NEM) was used
The effects of substrates, products and other ligands on the susceptibility ofinositol monophosphatase to proteolysis by endoprotease lys-C.
Gee NS
FEBS Journal, 284, 95-97 (1991)
Pavlo Pristatsky et al.
Analytical chemistry, 81(15), 6148-6155 (2009-07-14)
The hinge region of human IgG2 contains four cysteine residues involved in disulfide linkages between the heavy chains, as well as the heavy and light chains. These linkages provide the fundamental framework of three distinct IgG2 disulfide isoforms recently described.
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What amount of Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289, is present in the 3 unit package size?
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What is the molecular weight of Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289?
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The molecular weight is 30,000 Daltons (30 kDa).
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is the protease Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289, active in the presence of detergents?
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This enzyme is active in the presence of 1% Triton X-100 and also 0.1% SDS.
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