Fresh samples are preferred. If sample storage is required, proceed with homogenizing tissue or cell samples prior to storage. Store tissues and serum samples under uniform conditions at -80 °C for up to one month. However, long term storage may reduce the signal or activity. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/133/459/mak337bul.pdf
MAK337
Aconitase Assay Kit
sufficient for 100 colorimetric tests
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $467.00 |
About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 100 colorimetric tests
input
tissue homogenate(s)
cell lysate
serum
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; neurological disorders; rheumatological diseases
storage temp.
−20°C
General description
Aconitase (Aconitate Hydratase) is an enzyme in the citric acid (TCA) and glyoxylate cycles that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe⁴S⁴] 2+ cluster. Related diseases include aconitase deficiency (such as myopathy and exercise intolerance. Friedreich′s ataxia and diabetes.) Mitochondrial aconitase also plays a role in stabilizing mtDNA, thereby influencing mitochondrial gene expression. Aconitase serves as a reliable marker of mitochondrial and cellular oxidative stress. The alteration of mitochondrial aconitase can lead to reduced energy production. The cytosolic form can act as an aconitase and also as an iron regulatory protein. The enzyme′s activity is inhibited by citrate analogs and fluoracetate. Oxidative stress agents like peroxynitrite, hydrogen peroxide, and superoxide can deactivate the enzyme by converting the [Fe⁴S⁴] cluster to a [Fe3S⁴] cluster.
Application
Suitable for aconitase activity determination in biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
The Aconitase Assay Kit may be used for:
- Cellular and Mitchondrial Metabolism Research
- Oxidative Stress Research
- Neurological Research
Biochem/physiol Actions
The Aconitase Assay Kit measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.
Features and Benefits
- Fast and Sensitive Measurement: Achieve quantitative measurement of aconitase activity using just 20 µL of sample, with a detection range spanning from 0.5 to 100 U/L.
- High-Throughput Compatibility: Seamlessly integrate the kit into high-throughput handling systems, ensuring efficiency and accuracy in assays.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
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This Item | |||
|---|---|---|---|
| description sufficient for 100 colorimetric tests | description sufficient for 100 colorimetric tests | description sufficient for 100 colorimetric tests | description sufficient for 100 colorimetric tests |
| usage sufficient for 100 colorimetric tests | usage sufficient for 100 colorimetric tests | usage - | usage sufficient for 100 colorimetric tests |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| storage temp. −20°C | storage temp. - | storage temp. −20°C | storage temp. 2-8°C |
| input tissue homogenate(s) | input biological sample(s) | input - | input - |
| relevant disease(s) endocrinological disorders, diabetes; neurological disorders; rheumatological diseases | relevant disease(s) Alzheimer′s disease; Parkinson′s disease; diabetes; neurological disorders | relevant disease(s) neurological disorders | relevant disease(s) cardiovascular diseases; rheumatological diseases; neurological disorders; cancer; dermatological diseases |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
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Posso utilizar esse kit em amostras de tecido ou soro que já estavam congeladas no -80 ou apenas a fresco?
1 answer-
Helpful?
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Combien de cellules faut-il pour tester l'activité de l'aconitase? Est-il possible de réaliser le test directement sur un gel?
1 answer-
This kit protocol requires cells to be lysed. The cell count is not established and may vary by cell type. However, the sample weight required per test is 50mg. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/133/459/mak337bul.pdfHelpful?
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