The maximum sample volume per well is 50 ul. The SMAS concentration may vary broadly, based on sample type. The standard provided with this kit is diluted to a range of 0.078 - 5.0 milliunits per milliliter and 50 uL of each of the serially diluted standard is added to each well. For unknown samples, several sample dilutions are recommended. Please see the following link to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/415/788/mak152bul.pdf
MAK152
Colorimetric Sphingomyelinase Assay Kit
sufficient for 200 colorimetric tests
Synonym(s):
Neutral Sphingomyelinase Assay Kit
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $382.00 |
About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 200 colorimetric tests
detection method
colorimetric
relevant disease(s)
rheumatological diseases; cancer
storage temp.
−20°C
Gene Information
human ... SMPD2(6610), SMPD3(55512), SMPD4(55627)
mouse ... SMPD2(20598), SMPD3(58994), SMPD4(77626)
rat ... SMPD2(83537), SMPD3(94338), SMPD4(303790)
General description
Sphingomyelinase (SMase), or sphingomyelin phosphodiesterase, catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide, a second messenger involved in multiple cellular responses including the cellular response to stress. There are at least 5 types of SMases identified which are classified according to their cation dependence, cellular location, and pH optima for activity and include the acidic and neutral sphingomyelinase. Neutral SMases, along with the acidic SMases, are thought to be major contributors to the production of ceramide in response to cellular stress.
Application
This kit is suitable for the detection of sphingomyelinase activity in a variety of samples such as blood and cell extracts or for screening sphingomyelinase inhibitors and activators.
Biochem/physiol Actions
The Sphingomyelinase Assay Kit provides a simple and direct procedure for measuring neutral SMase activity in a variety of samples such as blood or cell extracts, or for screening SMase activators and inhibitors. SMase activity is determined by a coupled enzyme assay, which results in a colorimetric (655 nm) product proportional to the SMase activity present.
Features and Benefits
Compatible with high-throughput handling systems.
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This Item | |||
|---|---|---|---|
| description sufficient for 200 colorimetric tests | description sufficient for 100 fluorometric tests | description IgG fraction of antiserum | description affinity isolated antibody |
| Gene Information human ... SMPD2(6610), SMPD3(55512), SMPD4(55627) | Gene Information - | Gene Information human ... SMPD2(6610) | Gene Information human ... SMPD3(55512) |
| usage sufficient for 200 colorimetric tests | usage sufficient for 100 fluorometric tests | usage - | usage - |
| detection method colorimetric | detection method fluorometric | detection method - | detection method - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) rheumatological diseases; cancer | relevant disease(s) cancer | relevant disease(s) - | relevant disease(s) - |
Signal Word
Danger
Storage Class
10 - Combustible liquids
WGK
WGK 3
Hazard Codes
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Resp. Sens. 1
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