M6068

Anti-phospho-Myosin Light Chain (pSer¹⁹) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Anti-MLC
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD06411889
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 16

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: human (predicted), chicken
• technique(s): western blot: 1:1,000 using chicken gizzard myosin II phosphorylated in vitro by PAK
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer19)
• Gene Information: human ... MYL1(4632)

Description

General description

Myosin light chains belongs to the calmodulin (CaM) gene family.

Rabbit polyclonal anti-phospho-Myosin Light Chain (pSer19) antibody detects chicken MLC. Results show that reactivity with the antibody requires phosphorylation of MLC by active kinase, and that only the peptide corresponding to MLC (pSer19) blocks the antibody signal, thereby demonstrating the specificity of the antibody. Human (100% homologous) has not been tested, but is expected to react.

Immunogen

synthetic phosphopeptide derived from the region of chicken MLC that contains serine 19 (serine 20 including the initiating methionine). Human (100% homologous) has not been tested, but is expected to cross-react.

Application

Anti-phospho-Myosin Light Chain (pSer19) antibody produced in rabbit has been used in the detection of phosphorylated myosin: in human lung fibroblasts and avian lens cells by immunoblotting by immunohistochemical detection in rabbit aorta samplesby immunofluorescence detection in human submandibular gland tissues

Biochem/physiol Actions

Myosin light chain is a subunit of the conventional myosins, e.g., myosin II. In smooth muscle and nonmuscle cells, conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however, myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19, the major phosphorylation site and the preferred site for MLCK, activates myosin motor activity under physiological conditions.

Myosin light chains functions to maintain integrity of myosin protein. Mutations in the myosin light chain is implicated in familial hypertrophic cardiomyopathy.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (± 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable