Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

M5257

Sigma-Aldrich

Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver

ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)

Synonym(s):

L-Malate:NADP+ oxidoreductase-(oxalacetate-decarboxylating), Malate Dehydrogenase (oxalacetate-decarboxylating), Malic Enzyme

Sign Into View Organizational & Contract Pricing

Select a Size

25 UNITS
$340.00
100 UNITS
$954.00

$340.00

Estimated to ship onJune 02, 2025

All Photos(1)

Select a Size

Change View
25 UNITS
$340.00
100 UNITS
$954.00

About This Item

CAS Number:
9028-47-1
Enzyme Commission number:
1.1.1.40 (BRENDA, IUBMB)
EC Number:
232-834-8
MDL number:
MFCD00131568
UNSPSC Code:
12352204
NACRES:
NA.54

$340.00

Estimated to ship onJune 02, 2025

biological source

chicken liver

form

ammonium sulfate suspension

specific activity

10-30 units/mg protein (modified Warburg-Christian)

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate. [1]

Application

Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. [2] It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken. [3]

Biochem/physiol Actions

Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation. [4]

Unit Definition

One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO2, and NADPH per min at pH 7.4 at 25 °C.

Physical form

Suspension in 2.9 M (NH4)2SO4 solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na4EDTA, pH 6.0

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
105K7042
032K7037
120K7000
030H8050
030H80507

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

Glutamic-Oxalacetic Transaminase from porcine heart Type I, ammonium sulfate suspension, 200-500 units/mg protein

Sigma-Aldrich

G2751

Glutamic-Oxalacetic Transaminase from porcine heart

Dietary manganese requirement of juvenile yellow catfish Pelteobagrus fulvidraco, and effects on whole body mineral composition and hepatic intermediary metabolism
Tan, X., et al.
Aquaculture (Amsterdam, Netherlands), 326, 68-73 (2012)
A R Ashton
Archives of biochemistry and biophysics, 345(2), 251-258 (1997-10-06)
NADP-malic enzyme (NADP-ME, EC 1.1.1.40) was purified to near-homogeneity from leaves of the C4 dicot Flaveria bidentis and shown to possess intrinsic NAD-dependent malic enzyme activity. The NAD-dependent activity is optimal at pH 7.5 and in the presence of Mn2+.
Synthesis and Degradation of Malic Enzyme in Chick Liver.
Silpananta, P
The Journal of Biological Chemistry, 264, 5754-5761 (1971)
Min Lian et al.
Applied biochemistry and biotechnology, 162(4), 935-941 (2009-12-08)
The present study focused on improving docosahexaenoic acid (DHA) production by Schizochytrium sp. through N-methyl-N-nitro-N-nitrisiguanidine treatment coupled with ultraviolet radiation based on the metabolic pathway analysis. The activity of glucose-6-phosphate dehydrogenase of the mutant was higher than the parent strain
Thomas P Brutnell et al.
The Plant cell, 22(8), 2537-2544 (2010-08-10)
C(4) photosynthesis drives productivity in several major food crops and bioenergy grasses, including maize (Zea mays), sugarcane (Saccharum officinarum), sorghum (Sorghum bicolor), Miscanthus x giganteus, and switchgrass (Panicum virgatum). Gains in productivity associated with C(4) photosynthesis include improved water and
View All Related Papers

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service