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M3633

4-Methylumbelliferyl β-D-glucopyranoside

β-glucosidase substrate, fluorogenic, ≥99% (HPLC), powder

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Synonym(s):

MUD

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100 mg

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About This Item

Empirical Formula (Hill Notation):
C16H18O8
CAS Number:
Molecular Weight:
338.31
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
242-736-7
MDL number:
Beilstein/REAXYS Number:
94672

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Product Name

4-Methylumbelliferyl β-D-glucopyranoside, β-glucosidase substrate

Quality Segment

assay

≥99% (HPLC)

form

powder

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 316 nm; λem 372 nm (pH 9.1), λex 360 nm; λem 449 nm (Reaction product)

storage temp.

−20°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16-/m1/s1

InChI key

YUDPTGPSBJVHCN-YMILTQATSA-N

Application

4-Methylumbelliferyl β-D-glucopyranoside has been used as substrate:
  • in glucosylceramidase β enzyme activity assay in lysosome-enriched fractions from primary hippocampal neurons
  • in β-glucosidase assay during yeast fermentation
  • to assay glucocerebrosidase 1 (GBA1)-related glucosidase activity in macrophage cell line (RAW)

Biochem/physiol Actions

4-Methylumbelliferyl β-D-glucopyranoside is a synthetic enzymatic substrate for glycosidase. It has been used as substrate for the β- glucosidase from enterococci.

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This Item
M7633M9659M3657
description

β-glucosidase substrate

description

≥98% (TLC)

description

≥98% (HPLC)

description

≥97% (HPLC)

assay

≥99% (HPLC)

assay

≥98% (TLC)

assay

≥98% (HPLC)

assay

≥97% (HPLC)

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

solubility

water: 50 mg/mL, clear, colorless to very faintly yellow

solubility

pyridine: 50 mg/mL, clear, colorless to faintly yellow

solubility

pyridine: 20 mg/mL, clear, colorless to faintly yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

fluorescence

λex 360 nm; λem 449 nm (Reaction product), λex 316 nm; λem 372 nm (pH 9.1)

fluorescence

λex 360 nm; λem 449 nm (Reaction product), λex 317 nm; λem 374 nm (pH 9.1)

fluorescence

-

fluorescence

λex 350 nm; λem 375 nm (pH 7.0), λex 360 nm; λem 449 nm (Reaction product)


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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