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M3440

Sigma-Aldrich

Mutanolysin Assay Substrate

lyophilized suspension of washed Enterococcus faecalis STF-3 (ATCC 12984) cell walls

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.28

product name

Mutanolysin Assay Substrate, (Lyophilized suspension of washed Enterococcus faecalis STF-3 (ATCC 12984) cell walls.)

Quality Level

200

form

(Lyophilized suspension of washed Enterococcus faecalis STF-3 (ATCC 12984) cell walls.)

packaging

vial of 10 assays

shipped in

wet ice

storage temp.

−20°C

Application

The activity of mutanolysin can be determined by hydrolysis of the rehydrated cell suspension in a continuous spectrophotometric assay.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Yuan Qiao et al.
Nature chemical biology, 13(7), 793-798 (2017-05-30)
Peptidoglycan is an essential crosslinked polymer that surrounds bacteria and protects them from osmotic lysis. β-lactam antibiotics target the final stages of peptidoglycan biosynthesis by inhibiting the transpeptidases that crosslink glycan strands to complete cell wall assembly. Characterization of transpeptidases
Jumpei Uchiyama et al.
Applied and environmental microbiology, 77(2), 580-585 (2010-11-26)
In bacteriophage (phage) therapy against Gram-positive bacteria, such as Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis, members of a genus of SPO1-like viruses are typically employed because of their extreme virulence and broad host spectrum. Phage φEF24C, which is a

Protocols

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products.

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