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L9776

Sigma-Aldrich

Leucine Aminopeptidase, microsomal from porcine kidney

suitable for protein sequencing, BioReagent, lyophilized powder

Synonym(s):

Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase

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About This Item

CAS Number:
9054-63-1
Enzyme Commission number:
3.4.11.2 (BRENDA, IUBMB)
EC Number:
232-942-5
MDL number:
MFCD00131501
UNSPSC Code:
12352200
NACRES:
NA.26

product line

BioReagent

form

lyophilized powder

packaging

vial of 50 μg

suitability

suitable for protein sequencing

application(s)

metabolomics
vitamins, nutraceuticals, and natural products

storage temp.

−20°C

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General description

Leucine Aminopeptidase (LAP) is a proteolytic enzyme. It is an amino acid hydrolase that catalyzes the hydrolysis of leucine residues from the amino-terminal of proteins or peptides. LAP is naturally present in the liver, kidney, lung, and serum.

Application

Leucine Aminopeptidase has been used:
  • in proteomics analysis
  • as a treatment for protein depolymerization reaction
  • to test its effect on the degradation of human recombinant IL-8

Biochem/physiol Actions

Leucine Aminopeptidase serves as a biomarker for drug-induced liver injury because in case of liver injury, cirrhosis, hepatitis, and other disorders serum LAP activity increases. Abnormal expression of LAP results in tumor cell proliferation, angiogenesis, and invasion, so it can also serve as a cancer-related biomarker.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

inhibitor

Product No.
Description
Pricing

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Simone Giaveri et al.
Advanced materials (Deerfield Beach, Fla.), 33(44), e2104581-e2104581 (2021-09-24)
The billion tons of synthetic-polymer-based materials (i.e. plastics) produced yearly are a great challenge for humanity. Nature produces even more natural polymers, yet they are sustainable. Proteins are sequence-defined natural polymers that are constantly recycled when living systems feed. Digestion
A Kehlen et al.
European cytokine network, 12(2), 316-324 (2001-06-12)
In rheumatic joints, high concentrations of interleukin-8 (IL-8) have been measured in synovial fluid and in pannus tissue. In both locations aminopeptidase N (APN)/CD13, an exopeptidase with reported activity towards IL-8 is also present. The surprising stability of IL-8 in
Yong Huang et al.
Analytical chemistry, 91(13), 8085-8092 (2019-05-31)
Drug-induced liver injury (DILI) is a frequent cause of hepatic dysfunction as well as the single most frequent reason for removing approved medications from the market, and multispectral optoacoustic tomography (MSOT) is an emerging and noninvasive imaging modality for diagnosing
Fluorescent probes for detection and bioimaging of leucine aminopeptidase
Y Chen
Materials today chemistry, 15, 100216-100216 (2020)
Juan Reguera et al.
PLoS pathogens, 8(8), e1002859-e1002859 (2012-08-10)
The coronaviruses (CoVs) are enveloped viruses of animals and humans associated mostly with enteric and respiratory diseases, such as the severe acute respiratory syndrome and 10-20% of all common colds. A subset of CoVs uses the cell surface aminopeptidase N
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