L2650
Lectin from Glycine max (soybean)
peroxidase conjugate, lyophilized powder
Synonym(s):
SBA, Soybean agglutinin
About This Item
Recommended Products
biological source
Glycine max (soybean)
conjugate
peroxidase conjugate
assay
85.00-100.00% (Warburg-Christian)
form
lyophilized powder
potency
<80 μg per mL agglutination activity
peroxidase activity
25-100 units/mg protein
composition
Protein, ~95% modified Warburg-Christian
technique(s)
western blot: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
soybean ... LE1(100818710)
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Application
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
Unit Definition
Physical form
Preparation Note
Analysis Note
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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